Sall,Notl-Xhol-His6-stopNdel-His8-MBP-TEV-Sall-His8-MBP-V79pBAD-V29HpBADforpBADrevAmpNotlTEVSall,Notl-stopHAtag-Sall-EcoRI-V80pCMV-HApCMVM13R-20HAtagAmpSall,Notl-NotlstopNdel-MBP-TEV-Sall-V81pBAD-V30MBP-TEVpBADforSall,Notl-pBADrevAmpNotlXhol-His6-stopNdel-His8-MBP-TEV-Sall-V82pBAD-V29HSHis-MBP-TEVpBAD forpBADrevAmpNotlSall,Notl-strep-stopNdel-Strep-TEV-EcoRI-BamHI-V83MCSStrep-TEVpASK-17pluspASkforpASKrevAmpXhol-Sall-Ncol-EcoRV-HindNdel-His8-GB1-TEV-Sall-His8-GB1-V84pASK-V3pASkforpASK revAmpTEVSall,Notl-NotlstopNdel-MBP-Sall-V85Sall,Notl-MBPpASk forpASK-V28EpASKrevAmpNotlXhol-His6-stopNdel-His8-MBP-TEV-Sall-V86pASK-V29HHis-MBP-TEVpASkforpASKrevAmpSall,Notl-NotlstopNdel-MBP-TEV-Sall-V87pASK-V30Sall,Notl-MBP-TEVpASk forpASK revAmpNotlXhol-His6-stopNdel-His8-MBP-TEV-Sall-V88pASK-V29HSHis-MBP-TEVpASKrevpASkforAmpSall,Notl-NotlStrep-stopNdel-InfB21-T7T7Sall-V89pET30aeGFP-InfB21-eGFPKanNotlPromoterTerminatorSall,Notl-stopV90Ndel-Sall-InfB21-eGFPT7T7pET30aKanNotlInfB21-PromoterTerminatoreGFP-TEV-
SalI,NotIXhoI-His6- stop V79 pBAD-V29H NdeI-His8- MBP-TEVSalI,NotIstop SalINotI His8-MBPTEV pBAD for pBAD rev Amp V80 pCMV-HA HAtagEcoRISalI,NotIstop SalINotI HAtag pCMV M13R-20 Amp V81 pBAD-V30 NdeIMBP-TEVSalI,NotIXhoI-His6- stop SalINotI MBP-TEV pBAD for pBAD rev Amp V82 pBAD-V29HS NdeI-His8- MBP-TEVSalI,NotIstrep-stop SalINotI His-MBP-TEV pBAD for pBAD rev Amp V83 pASK-17plus NdeIStrep-TEVEcoRIBamHIXhoI-SalINcoIEcoRVHindIII MCS Strep-TEV pASk for pASK rev Amp V84 pASK-V3 NdeI-His8- GB1-TEVSalI,NotIstop SalINotI His8-GB1- TEV pASk for pASK rev Amp V85 pASK-V28E NdeIMBPSalI,NotIXhoI-His6- stop SalINotI MBP pASk for pASK rev Amp V86 pASK-V29H NdeI-His8- MBP-TEVSalI,NotIstop SalINotI His-MBP-TEV pASk for pASK rev Amp V87 pASK-V30 NdeIMBP-TEVSalI,NotIXhoI-His6- stop SalINotI MBP-TEV pASk for pASK rev Amp V88 pASK-V29HS NdeI-His8- MBP-TEVSalI,NotIStrep-stop SalINotI His-MBP-TEV pASk for pASK rev Amp V89 pET30a NdeIInfB21- eGFPSalI,NotIstop SalINotI InfB21-eGFP T7 Promoter T7 Terminator Kan V90 pET30a NdeIInfB21- eGFPTEVSalINotI InfB21-eGFP T7 Promoter T7 Terminator Kan
Sall,Notl-stopNdel-His8-eGFP-T7T7Sall-His8-eGFP-V91TEV-KanpET30aNotlTEVPromoterTerminatorSall,Notl-stopNdel-T7T7SUMO-Sall-V92SUMOKanpET30aSall,Notl-NotlPromoterTerminatorStrep-stopNdel-His8-MBP-TEV-T7T7Sall-His8-MBP-V93pET30aGFP-KanNotlTEV-GFPPromoterTerminatorSall,Notl-Strep-stopNdel-VHb-T7T7Sall-V94VHbpET30aSall,Notl-KanNotlPromoterTerminatorStrep-stopNdel-Tag2-Sall-T7T7V95Sall,Notl-Tag2KanpET30aNotlPromoterTerminatorStrep-stopNdel-MBP-T7T7Sall-V96Sall,Notl-MBPpET30aKanNotlPromoterTerminatorXhol-His6-stopNdel-T7T7MBP-Sall-V97MBPpET30aKanSall,Notl-NotlPromoterTerminatorStrep-stopEcoRV-SPNdel-His8-Sall-V98pFabSP-His8unknownunknownAmpSall,Notl-NotlStrep-stopNdel-MBP-Sall-T7T7V99MBPpET30aSall,Notl-KanNotlPromoterTerminatorXhol-His6-stopNdel-MBP-T7T7Sall-V100MBPpET30aSall,Notl-KanNotlPromoterTerminatorXhol-His6-stopNdel-GlyA-Sall-T7T7V101pET30aSall,Notl-GlyAKanNotlPromoterTerminatorXhol-His6-stopNdel-GacH-Sall-T7T7V102pET30aSall,Notl-GacHKanNotlPromoterTerminatorXhol-His6-stopNdel-XBP1-T7Sall-T7V103XBP1pET30aSall,Notl-KanNotlPromoterTerminatorXhol-His6-stop
SalI,NotIstop V91 pET30a NdeI-His8- eGFPTEVSalI,NotIstop SalINotI His8-eGFPTEV T7 Promoter T7 Terminator Kan V92 pET30a NdeISUMOSalI,NotIStrep-stop SalINotI SUMO T7 Promoter T7 Terminator Kan V93 pET30a NdeI-His8- MBP-TEVGFPSalI,NotIStrep-stop SalINotI His8-MBPTEV-GFP T7 Promoter T7 Terminator Kan V94 pET30a NdeI-VHbSalI,NotIStrep-stop SalINotI VHb T7 Promoter T7 Terminator Kan V95 pET30a NdeI-Tag2- SalI,NotIStrep-stop SalINotI Tag2 T7 Promoter T7 Terminator Kan V96 pET30a NdeIMBPSalI,NotIXhoI-His6- stop SalINotI MBP T7 Promoter T7 Terminator Kan V97 pET30a NdeIMBPSalI,NotIStrep-stop SalINotI MBP T7 Promoter T7 Terminator Kan V98 pFab EcoRV-SPNdeI-His8- SalI,NotIStrep-stop SalINotI SP-His8 unknown unknown Amp V99 pET30a NdeIMBPSalI,NotIXhoI-His6- stop SalINotI MBP T7 Promoter T7 Terminator Kan V100 pET30a NdeIMBPSalI,NotIXhoI-His6- stop SalINotI MBP T7 Promoter T7 Terminator Kan V101 pET30a NdeIGlyASalI,NotIXhoI-His6- stop SalINotI GlyA T7 Promoter T7 Terminator Kan V102 pET30a NdeIGacHSalI,NotIXhoI-His6- stop SalINotI GacH T7 Promoter T7 Terminator Kan V103 pET30a NdeIXBP1- SalI,NotIXhoI-His6- stop SalINotI XBP1 T7 Promoter T7 Terminator Kan