Pepsin crystals X90) Northrop, 1930) Northrop,J.H.(1930) “Crystallin pepsin,.l: Isolation and tests of purity"J.Gen.Physiol. 13:739-766
Pepsin crystals (X90) Northrop, 1930) Northrop, J. H. (1930) “Crystallin pepsin, 1: Isolation and tests of purity” J. Gen . Physiol. 13:739-766
(2) 酶学理论研究 >1894年，Fisher 锁钥学说。 >1958年，Koshland-一一诱导契合学说。 >1902年，Henri-一一中间产物学说。 >l913年，Michaelis和Menten-一一米氏学说。 >1960年，Jacob和Monod-一一操纵子学说
（2） 酶学理论研究 ➢1894年，Fisher——锁钥学说。 ➢1958年， Koshland——诱导契合学说。 ➢1902年，Henri——中间产物学说。 ➢1913年， Michaelis 和 Menten——米氏学说。 ➢1960年，Jacob 和 Monod——操纵子学说
Characteristics of enzyme active sites >Lock and key model(锁钥) 1894 picture by Emil fisher. This model assumed that only a substrate of the proper shape could fit with the enzyme. >Induced-fit model(诱导契合) > proposed by丹尼尔·科什兰 Daniel Koshland in 1958. >This model assumes continuous changes in active site structure as a substrate binds
Characteristics of enzyme active sites ➢Lock and key model (锁钥) 1894 picture by Emil fisher. This model assumed that only a substrate of the proper shape could fit with the enzyme. ➢Induced –fit model (诱导契合) ➢ proposed by 丹尼尔·科什兰 Daniel Koshland in 1958. ➢ This model assumes continuous changes in active site structure as a substrate binds
Daniel E.Koshland,Jr. Professor in the Graduate School (Division of Biochemistry&Molecular Biology)
O 0 ES 中间产物学说 s P 三
中间产物学说 ES S P E