Binding Catalytic site site Enzyme Substrate Formation of the enzyme-substrate complex First step in an enzyme catalyzed reaction + S+一ES Enzyme Substrate Complex
Formation of the transition state An intermediate species is then formed. ES E砖 note change
Formation of the product The product is finally made and the enzyme is ready for another substrate. EP E+P
米凯利斯门顿 Leonor Michaelis Maud Menten 1875-1949 1879-1960
米凯利斯-门顿
CtgavmAAtlnenttT 麦Vmax Km Substrate concentration,[S](mM) Vo Vmax[S] Km+[S] Michaelis-Menten equation
Michaelis-Menten equation