Extraction of crude fats ethyl ether in acid(酸性乙 醚提取法) Principle: > sample +HCI +H20 Hydrolysis by heatingreleased associated fats to exist in the free form. >Hydrolyzed sample +ethanol +ethyl ether +petroleum ether Extract >Crude fats >Recycle solvents,and weight
Extraction of crude fats ethyl ether in acid(酸性乙 醚提取法） Principle: ➢ sample +HCl +H2O released associated fats to exist in the free form. ➢ Hydrolyzed sample +ethanol +ethyl ether +petroleum ether Crude fats ➢ Recycle solvents, and weight Hydrolysis by heating Extract
Procedure >Sample preparation >Hydrolysis heating at 70-80C in a water bath for 40-50min Extract ethanol ethyl ether,petroleum ether, >Weighting >Calculating
Procedure ➢ Sample preparation ➢ Hydrolysis heating at 70-80℃ in a water bath for 40-50min ➢ Extract ethanol , ethyl ether, petroleum ether, ➢ Weighting ➢ Calculating
Attention: sample without high saccharides and phospholipids Sample include:liquid sample,semi-solid sample, sample difficult to be dried,and sample that can not to be analyzed by soxhlet extractor. >It can determine the associated fats in foods ethanol can make protein precipitated,retard emulsion,promote fats together,and solute carbonhydrates. >Addition of petroleum ether can decrease the solubiltiy of ethanol in ethyl ether,and favorable for phase separation
Attention: ➢ sample without high saccharides and phospholipids ➢ Sample include: liquid sample, semi-solid sample, sample difficult to be dried, and sample that can not to be analyzed by soxhlet extractor. ➢ It can determine the associated fats in foods. ➢ ethanol can make protein precipitated, retard emulsion, promote fats together, and solute carbonhydrates. ➢ Addition of petroleum ether can decrease the solubiltiy of ethanol in ethyl ether, and favorable for phase separation
Extraction of crude fats by ethyl ether in alkali( 性乙瞇提取法)（罗兹一哥特里法） Principle >Sample NH3.H20+ethanol Hydrolysis Released associated fats in foods >Hydrolyzed sample +ethyl ether +petroleum ether extract fats >Recycle solvents,dried,and weighting
Extraction of crude fats by ethyl ether in alkali(碱 性乙醚提取法）（罗兹－哥特里法） Principle ➢Sample + NH3.H2O+ ethanol Hydrolysis Released associated fats in foods ➢Hydrolyzed sample +ethyl ether +petroleum ether extract fats ➢Recycle solvents, dried, and weighting
Procedure >Adding NH3.H2O into sample,mixed,and heated for 5min in a 60C water bath. >Adding ethanol to reaction system,mixed and cooled. Extraction with ethyl ether and petroleum ether. >Recycle solvents,dried at 100-105C,and weighting
Procedure ➢ Adding NH3.H2O into sample, mixed, and heated for 5min in a 60℃ water bath. ➢ Adding ethanol to reaction system, mixed and cooled. ➢ Extraction with ethyl ether and petroleum ether. ➢ Recycle solvents ,dried at 100-105℃， and weighting