Determination of fats in food >Soxhlet extractor method >Extraction of crude fats ethyl ether in acid(酸性乙瞇提取法) Extraction of crude fats by ethyl ether in Akai(碱性乙醚提取法)（罗兹一哥特里法） >Babcock Method and Gerber method(巴布科氏法和盖勃氏法) >Extraction of fats by Chloroform-Methanol(氯仿-甲醇抽提法)
Determination of fats in food ➢ Soxhlet extractor method ➢ Extraction of crude fats ethyl ether in acid(酸性乙醚提取法） ➢ Extraction of crude fats by ethyl ether in Alkali (碱性乙醚提取法）（罗兹－哥特里法） ➢ Babcock Method and Gerber method(巴布科氏法和盖勃氏法) ➢ Extraction of fats by Chloroform-Methanol (氯仿－甲醇抽提法）
索氏提取法 Principle 冷凝器 样品 乙醚或石油醚 蒸去溶剂 粗脂肪 提取管 虹吸管 联接管 提取瓶 图1索氏抽提器
索氏提取法 样品 乙醚或石油醚 蒸去溶剂 粗脂肪 Principle
Procedure: 1)Preparation of sample: Solid sample-grinding Semi-solid sample or liquid sample-adding sand- dried-grinding 2)Preparation of filter paper tube 3)Put sample ready into the filter paper tube. 冷凝罗 4)Extract the fats with solvents 5)Weighting 提取管 虹吸管 6)Calculation 联接管 提取瓶 图1索氏抽提器
Procedure: 1)Preparation of sample: Solid sample –grinding Semi-solid sample or liquid sample– adding sand– dried—grinding 2) Preparation of filter paper tube 3) Put sample ready into the filter paper tube. 4) Extract the fats with solvents 5) Weighting 6) Calculation
Attention 1) Crude fats include phospholipids,pigments,wax, volatile oil,etc. 2) Sample should contain greater amount of free fats,little associated fats. 3) Samples should be dried(decrease the efficiency. increase nonfat substance) 4) If more dextrin or saccharide is included in sample,it should be remove by water 5) Ethyl ether should be purified:no water,no peroxide,no alcohol. 6) It should be heated in water bath,be careful of firing
Attention 1) Crude fats include : phospholipids, pigments,wax, volatile oil, etc. 2) Sample should contain greater amount of free fats, little associated fats. 3) Samples should be dried(decrease the efficiency, increase nonfat substance) 4) If more dextrin or saccharide is included in sample, it should be remove by water 5) Ethyl ether should be purified: no water, no peroxide, no alcohol. 6) It should be heated in water bath, be careful of firing
Determination of fats in food Soxhlet extractor method Principle 冷凝器 Ethyl ether or sample Petroleum ether Distill Crude fat 提取管 虹吸管 联接管 提取瓶 图1索氏抽提器
Determination of fats in food Soxhlet extractor method sample Distill Crude fat Ethyl ether or Petroleum ether Principle