Chapter 11 Vitamins Analysis
Chapter 11 Vitamins Analysis
Introduction 1.What is vitamins? VITAMIN:VITA (LIFE)-AMINE 2.Vitamins classification Hydrosoluble vitamins:B,C, Liposoluble Vitamins:A,D,E,K 3.Vitamins and health
Introduction 1. What is vitamins? VITAMIN：VITA (LIFE)-AMINE 2. Vitamins classification Hydrosoluble vitamins: B, C, Liposoluble Vitamins: A, D, E, K 3. Vitamins and health
Vitamins determination The general techniques for determining vitamins can be used for biological liquids and plant or animal tissues. The general principles are the same,but the extraction methods differ depending on the matter being analyzed.For many decades,the determination of vitamins has been based on the evolution of diverse technologies from microbiological methods to more sophisticated techniques like liquid or gaseous phase chromatography coupled with specific detection systems(ultraviolet or fluorimetric). The real problem at present is no longer the methods of determination;rather,it is extraction procedures.The current change in legislation that permits vitamin supplement food substances necessitates constant analytical control,and underlines the importance of the methods for determining these vitamins
Vitamins determination The general techniques for determining vitamins can be used for biological liquids and plant or animal tissues. The general principles are the same, but the extraction methods differ depending on the matter being analyzed. For many decades, the determination of vitamins has been based on the evolution of diverse technologies from microbiological methods to more sophisticated techniques like liquid or gaseous phase chromatography coupled with specific detection systems(ultraviolet or fluorimetric). The real problem at present is no longer the methods of determination; rather, it is extraction procedures. The current change in legislation that permits vitamin supplement food substances necessitates constant analytical control, and underlines the importance of the methods for determining these vitamins
HC、CH3 CH, CH, H CH2OH CH CH OH CH. VA2 H.O CH, CH, CH CH CH B一胡萝卜素 R3 R2 HO 生育三烯酚 生育酚 H C H● H● H C CH. CH, 麦角固醇 CH HO HO 7 脱氢胆固醇 维生素D3(胆钙化醇)
CH2 CH3 CH3 CH3 CH3 CH2OH CH3 H C CH3 3 CH3 CH3 CH2OH CH3 CH3 CH3 CH3 CH3 CH3 CH3 CH3 H3C CH3 H3C C H3 C H3 HO C H3 C H3 H3C CH3 CH3 HO H3C H3C O CH3 CH3 CH3 CH3 H3C R1 R3 R2 HO O CH3 CH3 CH3 CH3 H3C R1 R3 R2 HO VA1 7－脱氢胆固醇 麦角固醇 生育三烯酚 生育酚 β－胡萝卜素 VA2 维生素D3 (胆钙化醇) H3C CH3 CH3 HO H3C H3C
Global Extraction Tech for Liposoluble Vitamins Weigh proper amount of the Add 10 ml of33%KOH and food substance and transfer to 40 ml ethanol a 150 ml round flask Cool very rapidly,then add 17 Boil at reflux for 30 mins ml of 25%HCI,and cool again Add 50 ml of petroleum ether Wait to obtain complete and shake vigorously for 3 separation of the two phases mins Evaporate to dry in a vacuum Remove and filter the organic at a temperature of 35 degree phase through anhydrous sodium phases Redissolve the residue with a know volume of hexane for HPLC analysis
Global Extraction Tech for Liposoluble Vitamins Weigh proper amount of the food substance and transfer to a 150 ml round flask Redissolve the residue with a know volume of hexane for HPLC analysis Evaporate to dry in a vacuum at a temperature of 35 degree C Remove and filter the organic phase through anhydrous sodium phases Wait to obtain complete separation of the two phases Add 10 ml of 33% KOH and 40 ml ethanol Boil at reflux for 30 mins Cool very rapidly, then add 17 ml of 25% HCl, and cool again Add 50 ml of petroleum ether and shake vigorously for 3 mins