Measurements of RDCs Frequency based methods(IPAP) rri iils 03 Waltz-16 g0 g1 g2 1g3 g5 94 isotropic anisotropic φ32x,2y,2(-x),2(y） φg:8x,8(-x) Orec:2x,2(-x) 18 φgof Add 119 In Phase 94.2Hz0 74.9Hz 120 g on 93.9Hz Subtract 84.6Hz 121 Anti Phase Residual Dipolar Couplings(RDC)are measured as 122 contributions to lines splittings in anisotropic solution relative577 to isotropic solution. 1H ppm
Measurements of RDCs Frequency based methods (IPAP) Residual Dipolar Couplings (RDC) are measured as contributions to lines splittings in anisotropic solution relative to isotropic solution
Jc的测定：脉冲序列2 IPAP HNCO(Ottiger et al..1998) H 1: 15N WALTZ 16 03 13C 13Ca Grad i的 G G2G3 G3G4 Gs GeG7 Gz F:(C) 76.6 ###Echo AntiEcho add: 1768 -Hi sin(2/CoNTC)sin(2/CoNTC)sin(ct1-Cotl) 1n. ###Echo AntiEcho sub: 172 177.4 +Hsin(2/CoNTC)sin(2/CoNTC)sin(ont1+cof1) 8.6 85 8.4 ppm F2(H)
JHNC΄的测定:脉冲序列2D IPAP HNCO(Ottiger et al. 1998)