生物大分子波谱学原理 吴季辉 7.1异核相关谱 蛋白质研究中用的异核相关谱均利用单键偶合，分为两类： HSQC (Heteronuclear Single-Quantum Coherence) Simple HSQC Decoupled HSQC Constant-time HSQC HMOC (Heteronuclear Multiple-Quantum Coherence)
7.1 异核相关谱 蛋白质研究中用的异核相关谱均利用单键偶合，分为两类： HSQC (Heteronuclear Single-Quantum Coherence) Simple HSQC Decoupled HSQC Constant-time HSQC HMQC (Heteronuclear Multiple-Quantum Coherence) 生物大分子波谱学原理 吴季辉
Modification of a refocused I-S INEPT into a 2D HSQC sequence,and use of States-TPPI quad detection INEPT 2-spin INEPT order 31 φ12 φ1=6-x φ2=x,x,-x,-x φ31=x-七-6x G: 2LS±21S2±2lS2 /4s φ31 φ2 φ1=6x +States-TPPI =1(0)+(ni-1)/sw1 2=xx,-x,-x G φ31=北，-6-x,x +States-TPPl 21S.21-S.2S,21Scos(os1)21S.215 ×cos(元J11) -21-Sx sin(os n) ×cos(πJt)
生物大分子波谱学原理 吴季辉 间接维正负频率区别 Experiment Increment Pulse phase Receiver phase TPPI (4+1) t1(0)+(4k)△ X X (4k+2) t1(0)+(4k+1)△ y x (4k+3) t1(0)十(4k+2)△ x x (4k+4) t1(0)+(4k+3)△ y X States (4k+1) t1(0)+(4k)2△ X (4+2) t1(0)+(4k)2△ y X (4k+3) t(0)+(4k+1)2△ X X (4+4) t1(0)计(4k+1)2△ TPPI-States (4k+1) t1(0)+(4k)2△ X (4k+2) t1(O)t(4)2△ y x (4k+3) t1(0)+(4k+1)2△ -X -X (4k+4) t1(0)+(4k+1)2△ y -X
间接维正负频率区别 生物大分子波谱学原理 吴季辉
Important Features of HSQC Spectra Start with 1H magnetization:gain YH/YN A factor of 10 in sensitivity Detect 1H magnetization:gain (YH /YN)2 Another factor of 100 Even though you must acquire 32-64 t1 points,far more sensitive than direct detection. 相干阶路线？
相干阶路线?
States quadrature:change the phase by 900 for each t increment to select cosine and sine components of S-spin magnetization 1.For each value of t, 14 4 4 31 acquire two FIDs which phase =1 are stored separately,cor- 1=6x 1=x,-术 responding to phase=1 2■x,-水，-x and phase=2. 31=,-x-， G 2.For the FID with phase=1,set 01=x,-x.This transfers 215.21.s 21.S es 21S es 21,S.cs ly cos(osh) IS cos(@st)to proton 21Sx ss -25x ss 2fSx ss (at the end of the se- 2I-S cs=cos(oos 11) quence ss =sin(os 1) 2.For the FID with phase=2,set 4 phase =2 4 31 1=y-y.This transfers IS sin(@st to proton 01=5-y =5少 (at the end of the se- 2=x,x-x,-x quence. φ31=x,--x,x 2S21S 21.S,cs -21Sxes 2hSxes 21-Sy ss 21-S:ss 2lxSzss ly sin(os f1)