RNAi的提出 ●1998，Fire et al ●材料：线虫 ●目的：同上 ·方法： 注射经过纯化的由T4和T7RNA聚合酶体外转录 得到的ss-RNA;注射经过纯化后的ds-RNA 结果： ss-RNA引起微弱的抑制作用； ds-RNA比前者高两个数量级的抑制效果
⚫ 1998，Fire et al ⚫ 材料：线虫 ⚫ 目的：同上 ⚫ 方法： 注射经过纯化的由T4和T7 RNA聚合酶体外转录 得到的ss-RNA; 注射经过纯化后的ds-RNA ⚫ 结果： ss-RNA 引起微弱的抑制作用； ds-RNA 比前者高两个数量级的抑制效果。 RNAi的提出
●分析： GuoS博士遇到的正义RNA抑制基因表达的现象 以及过去的反义RNA技术对基因表达的阻断，都 是由于体外转录所得RNA中污染了微量双链RNA 而引起。 该小组将这一现象称为RNA干扰(RNA interference,简称RNAi)。 Fire A,Xu S,Montgomery MK,Kostas SA,Driver SE,Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.Nature.1998 Feb 19;391(6669):806-11
⚫ 分析： Guo S博士遇到的正义RNA抑制基因表达的现象， 以及过去的反义RNA技术对基因表达的阻断，都 是由于体外转录所得RNA中污染了微量双链RNA 而引起。 该 小 组 将 这 一 现 象 称 为 RNA 干 扰 （ RNA interference， 简称RNAi）。 Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998 Feb 19;391(6669):806-11
Nature,1998,806-811-PubMed result-Microsoft Internet Explorer g日8 文件E)编辑日查着地收寐A)工具D帮助出 G后退③ Potent and specific genetic interference by double.[Nature.1998]-PubMed result-Microsoft Internet Explorer 白回® 地址D圈文件D编辑（日查看0收意(A)工具①帮助心 ⑧os0a ④后退·⊙·的日小P被素☆收寐夹的B·四·☐ NCBI http://www.ncbi.nlm.nh.gov/pubmed/9486653 )转到链接” Publ ③0900- 提未日书接·色·回·⑦问问、冥·Q1325℃音乐 卡到色山城夜景 S N NCBI Resources How To My NCBI Sign In Neman Display Se PublMed.gou Search:PubMed Limits Advanced search Help SearchClear Results RNA Display Setings:Abstract Send to nature☐ 1.Paln Cell E Nature.1998Feb19391(6559r806.-11 Related articles PMID Potent and specific genetic interference by double-stranded RNA in Caenorhabditis Relal elegans. Double-stranded RNA poses puzzle. OIM Fire A Xu 8,Montgomery MK Kostas SA Driver SE,Mello CC. [Nature.1998] e Specitic inhbition of gene expression by small Comment in: doubie-stranded RI[Proc Natl Acad SciUS A.2001] Nature.1998Feb.19.391(6669)744.5. Pote Fire Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an Natu endogenous gene.Such effects have been proposed to result from a simple antisense mechanism that depends on PMID hybridization between the injected RNA and endogenous messenger RNA,transcripts.RNA interference has been used in the See reviews.I See a. Relat nematode Caenorhabditis elegans to manipulate gene expression.Here we investigate the requirements for structure and delivery of the interfering RNA To our surprise,we found that double-stranded RNA was substantially more effective at Cited by over 100 PubMed Central articles producing interference than was either strand individually.After injection into adult animals,purified single strands had at most Display Se a modest effect,whereas double-stranded mixtures caused potent and specifc interference.The efects ofthis interference were evident in both the injected animals and their progery.Only a few molecules of injected double-stranded RNA were Differential localzetion and ndependent acauisition 完毕 required per affected cell,arguing against stochiometric interference with endogenous mRNA and suggesting that there coul of the H3K9me2 and H3K9me3 c[PLoS Genet.2010] be a catalytic or amplification component in the interference process. PMD:9486653 [PubMved-indexed for MEDLNE] See all. 完中 Internet
Discovery of RNAi Nature1998391:806-811 Double-stranded RNA Neg.control Uninjected B antisense inject C.elegans Antisense RNA dsRNA Mex-3 mRNA detection in embryos by in situ hybridization
Discovery of RNAi Double-stranded RNA inject C. elegans Neg. control Uninjected Antisense RNA dsRNA sense antisense Nature 1998 391:806-811 Mex-3 mRNA detection in embryos by in situ hybridization
《Science》杂志评为2001年的十大科学进展之一，并名列2002 年十大科学进展之首。2006年10月2日瑞典卡罗林斯卡医学院宣 布，将2006年诺贝尔生理学，医学奖授予两名美国科学家安德 鲁·菲尔和克雷格·梅洛，以表彰他们发现了RNA干扰现象。 易邦来频道 Craig C.Mello,PhD 编易探索预道 ec165.com/oo
《Science》杂志评为2001年的十大科学进展之一，并名列2002 年十大科学进展之首。2006年10月2日 瑞典卡罗林斯卡医学院宣 布，将2006年诺贝尔生理学，医学奖授予两名美国科学家 安德 鲁·菲尔和 克雷格·梅洛，以表彰他们发现了RNA干扰现象