意外发现 P35S 色素基因 ●1990，Jorgensen et al, 重组 ●材料：矮牵牛花(petunias)) Ti质粒 十色素 ●目的：得到紫色更深的花 基因 ●方法：构建强启动子色素基因（苯基苯乙 转化 烯酮合酶)表达载体，导入牵牛花 ·结果：杂色（花变得更白）。 ·分析：所转基因（外源）和同源的内源基 因的表达都被抑制。这个现象称之为共抑制 繁殖 (co-suppression)
⚫ 1990, Jorgensen et al, ⚫ 材料：矮牵牛花( petunias) ⚫ 目的：得到紫色更深的花 ⚫ 方法：构建强启动子色素基因（苯基苯乙 烯酮合酶）表达载体，导入牵牛花 ⚫ 结果：杂色（花变得更白）。 ⚫ 分析：所转基因（外源）和同源的内源基 因的表达都被抑制。这个现象称之为共抑制 （co-suppression） P35S 色素基因 Ti质粒 ＋色素 基因 重组 转化 繁殖 意外发现一
Co-suppression was first discovered in petunias in 1990 by Richard Jorgensen
意外发现二 ●1995，康乃尔大学Guo Su et al ●材料：秀丽新小杆线虫(C.elegans) ●目的：消除线虫第一次分裂的不对称性 。方法： 反义RNA特异性地阻断par-1基因的表达； 正义RNA以期观察到基因表达的增强
⚫ 1995,康乃尔大学Guo Su et al ⚫ 材料：秀丽新小杆线虫（C. elegans） ⚫ 目的：消除线虫第一次分裂的不对称性 ⚫ 方法： 反义RNA 特异性地阻断par-1基因的表达； 正义RNA 以期观察到基因表达的增强。 意外发现二
结果：二者都同样地切断了par-1基因的表达途径。 分析：无法解释。 这与传统上对反义RNA技术的解释不相符合。该研究 小组一直没能给这个意外以合理解释。 Guo S,Kemphues KJ.par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed.Cell.1995 May 19;81(4):611-20
结果: 二者都同样地切断了par-1基因的表达途径。 分析：无法解释。 这与传统上对反义RNA技术的解释不相符合。该研究 小组一直没能给这个意外以合理解释。 Guo S, Kemphues KJ. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell. 1995 May 19;81(4):611-20
文件E编(E)查若仙收藏(A工具①帮助山 ④后温，③·8日公P搜索☆收旅夹⊙公·品■，□ 地址D地址D图hkp:ww,ncti.nln.nh.ovises/ere )转到链接” ⑤osd Soso@ y搜索量书签，@，圆，③问问，厚，013250了音乐 中食山城夜景 名N 号NCBI Resources巴How To回 MyNCBI Sign in PU地t址o图p:w.bi.ntm.nh.ovpbmed7581i5 可习转到摆 U.S.Na ③0300 提素0书签·鱼·回·⑦问时，属·●1325心心音乐 十绿具山城瓶景 号NCBI Resources How To My NCBI Sign in R Displa Pub Med.gov Search:PubMed Limits Advanced search Help U.S.National Lbrary of Medicine Search Clear Are y National institutes of Heath F2R Display Settings:Abstract 8endt地可 Cell Press P s Ce.1995Msy1981(41611-20. Related articles G Resu par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed. Guo S,Kemphues KJ Section of Genetics and Development,Comell universty,hac New York 14853.USA A non-muscle myosin required for embryonic E polarity in Caenorhabditis elegans.[Nature.1996 The first cleavage of C.elegans is asymmetric,generating daughter cells with different sizes,cytoplasmic components,and T fates.Mutations in the par-1 gene disrupt this asymmetry.We report here that par-1 encodes a putative Sen/Thr kinase with The PAR proteins:fundamental players in anima cell polarization. [Dev Cel.2007 D similarity to kinases from yeasts and mammals.Two strong alleles have mutations in the kinase domain,suggesting that Re kinase activity is essential for par-1 function.PAR-1 protein is localized to the posterior perphery of the zygote and is Te PAR-PKC system cell Sci.2006 distributed in a polar fashion preceding the asymmetric divisions ofthe germline lineage.Because PAR-1 distribution in the germline correlates with the distribution of germline-specific P granules,it is possible that PAR-1 functions in germline See reviews.See all. 2 development as well as in establishing embryonic polarity Cited by 93 PubMed Central articles PMD:7758115 [PubMed indexed for MEDLNE] Cellular Symmetry Breaking during Caenorhabdtis 凸完毕 Publicaion Types MeSHTerms Substances condaryourc Suppor elegans Deve[Cold Spring Harb Perspect Biol.2009 Widely conserved signalng pathways in the LinkOut-more resources establshment[Cold Spring Harb Perspect Bi 2009 图完毕 See al. 完毕 Internet