BIOCHEMISTRYANDMOLECULARBIOLOGYOrganizationofthechainThe inner mitochondrial membrane can be disrupted intofive separate enzyme complexes, called complex I、IIIII、 IV and V.Complexes I to IV each contain part of the electrontransport chain, whereas complex V catalyzes ATPsynthesis.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Organization of the chain The inner mitochondrial membrane can be disrupted into five separate enzyme complexes, called complex Ⅰ、Ⅱ、 Ⅲ、Ⅳ and Ⅴ. Complexes Ⅰto Ⅳ each contain part of the electron transport chain, whereas complex Ⅴ catalyzes ATP synthesis
Respiratory chainNADH工+H.ONADH-QNADQH2redreductaseUbiquinoneCytochromeCytochromeCytochromereductaseoxidaseaSuccinate-QreductaseSuccinateFumarateRespiratory chain
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Respiratory chain
BIOCHEMISTRYANDMOLECULARBIOLOGYComplex I :NADH-Co Q reductaseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Complex Ⅰ : NADH-Co Q reductase
BIOCHEMISTRYANDMOLECULARBIOLOGYFormationof NADH:NAD+is reduced toNADHbydehydrogenaseswhich remove two hydrogen atoms form their substrate.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Formation of NADH: NAD+ is reduced to NADH by dehydrogenases which remove two hydrogen atoms form their substrate
BIOCHEMISTRYANDMOLECULARBIOLOGYNADHdehydrogenase:the free proton plus the hydride ion carried byNADH are next transferred to NADHdehydrogenase, an enzyme complex embedded inthe innermitochondrialmembraneDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY NADH dehydrogenase: the free proton plus the hydride ion carried by NADH are next transferred to NADH dehydrogenase, an enzyme complex embedded in the inner mitochondrial membrane