Massachusetts Institute of Technology RT Harvard medical school Brigham and Womens/Massachusetts General Hosp VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J CELL-MATRIX INTERACTIONS M. Spector, Ph. D
Massachusetts In Massachusetts Institute of Technology stitute of Technology Harvard Medical School Harvard Medical School Brigham and Wo Brigham and Women’s/Massachusetts General Hosp. men’s/Massachusetts General Hosp. VA Boston Healthcare System VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J 2.79J/3.96J/BE.441/HST522J CELL-MATRIX INTERACTIONS MATRIX INTERACTIONS M. Spector, Ph.D. M. Spector, Ph.D
Articular Cartilage Extracellular Matrix Cell 4 mm e 10 um
Cell Extracellular Matrix 4 mm 10 µm Articular Cartilage
Chondrocytes(p2 canine) in a Type I Collagen-GAG Scaffold Image removed due to copyright considerations “ Control volume B. Kinner
Chondrocytes (P2 Canine) in a Type I Collagen-GAG Scaffold Image removed due to copyright consi derati ons. “Control Volume” B. Kinner
UNIT CELL PROCESSES Concept of a“ Control volume” around a cell Soluble Regulator Cell Matrix Product+ Soluble (Regulator) Regulator b “ Control volume
UNIT CELL PROCESSES UNIT CELL PROCESSES Concept of a “Control Volume” around a Cell Concept of a “Control Volume” around a Cell Cell + Matrix Cell + Matrix Product + Soluble Product + Soluble (Regulator) (Regulator) Regulator B Regulator B “Control Volume” “Control Volume” Soluble Soluble Regulator Regulator A
COLLAGEN-GAG MATRICES: MODEL BIOMATERIALS (ANALOGS OF EXTRACELLULAR MATRIX) Investigation of cell interactions (UCPs)in vitro Type I(bovine and porcine) Type II(porcine) Image removed due to Chondroitin 6-sulfate copyright considerations Imm Image removed due to copyright considerations Freeze-dried Dehydrothermally cross-linked Additional cross-linking 500um IV Yannas, et aL. PNas. 1989
COLLAGEN-GAG MATRICES: MODEL BIOMATERIALS (ANALOGS OF EXTRACELLULAR MATRIX) Investigation of cell interactions (UCPs) in vitro • Type I (bovine and porcine) • Type II (porcine) • Chondroitin 6-sulfate Image removed due to copyright consi derati ons. 1mm Image removed due to copyright consi derati ons. • Freeze-dried • Dehydrothermally cross-linked • Additional cross-linking 500 µ m IV Yannas, et al. PNAS, 1989