Massachusetts Institute of Technology yE图 Harvard medical school Brigham and women's hospital VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J INTEGRINS I.V.Yannas, Ph. D, and M. spector, Ph.D
Massachusetts In Massachusetts Institute of Technology stitute of Technology Harvard Medical School Harvard Medical School Brigham and Wo Brigham and Women’s Hospital men’s Hospital VA Boston Healthcare System VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J 2.79J/3.96J/BE.441/HST522J INTEGRINS INTEGRINS I.V. Yannas, Ph.D. and M. Spector, Ph.D. I.V. Yannas, Ph.D. and M. Spector, Ph.D
UNIT CELL PROCESSES Regulator UCP Cell Matrix Product Regulator Connect ECM Mitosis Tiss Adhesion ynthesis Epithelia Protein Migration Muscle Collagen Contraction Nerve Biomaterial Endocytosis Integrin Exocytosis
UNIT CELL PROCESSES UNIT CELL PROCESSES Cell + Matrix Product + Regulator Cell + Matrix Product + Regulator Regulator Regulator Mitosis Mitosis Synthesis Synthesis Migration Migration Contraction Contraction Endocytosis Endocytosis Exocytosis Exocytosis UCP Connect. Connect. Tiss. Epithelia Epithelia Muscle Nerve ECM Adhesion Adhesion Protein Protein Collagen Collagen Biomaterial Biomaterial Integrin Integrin
The cell and its membrane molecules Plasma membrane -5nm Rough ER Smooth Er Nucleus -3-10 um Nucleolus Nuclear pore Golgi complex Extracellular Cilia Oligosaccharide Glycoprotein Peripheral Glycolipid Integral 1 um Mitochondrian hydrophobic cytoskeletal fibers Hydrophilic Intracellular
The Cell and Its Membrane Molecules
CELL ADHESION MOLECULES Type Cell-Matrix ell-Cell Integrin Homophilic N-CAM Cadherin Heterophilic Integrins bind to adhesion proteins and some to collagen
CELL ADHESION MOLECULES CELL ADHESION MOLECULES Type Cell -Matrix Cell -Cell Integrin Integrin * √ √ Homophilic Homophilic • N -CAM • Cadherin Cadherin √ Heterophilic Heterophilic √ * Integrins Integrins bind to adhesion proteins and some to collagen bind to adhesion proteins and some to collagen
Ligand binding After sM albelda. cA Buck eglon FASEB J.,4:2868(1990) β subunit Ca* a subunit Schematic of a typical integrin s S-S I Xe Extracellular The RGD*amino acid sequence on domain Membrane adhesion proteins(e.g, fibronectin)was identified as the integrin-binding region (i.e, the ligand for integrin receptors) Tatin E Ruoslathi and md pierschbacher Vinculin Sci,238:491(1987) a-actinin arginine-glycine-aspartic acid Actin cytoskeleton
After SM Albelda, CA Buck, FASEB J., 4:2868 (1990) Schematic of a typical integrin The RGD* amino acid sequence on adhesion proteins (e.g., fibronectin) was identified as the integrin-binding region (i.e., the ligand for integrin receptors) – E Ruoslathi and MD Pierschbacher, Sci., 238:491 (1987) * arginine-glycine-aspartic acid