Massachusetts Institute of Technology Harvard Medical School Brigham and Womens Hospital VA Boston Healthcare System 2.79J/3.96J/BE44I/HST522J BIOMATERIALS-TISSUE INTERACTIONS Tools"for Understanding the Molecular, Cellular, and Physiological, Bases of the Tissue Response to Implants M. Spector, Ph D. and I.V. Yannas, Ph. D BIOMATERIALS-TISSUE INTERACTIONS Tissue* Biomaterials Cell Matrix* Structure comprising cells of the same type 会 Solid surface Page 1
2.79J/3.96J/BE.441/HST522J BIOMATERIALS-TISSUE INTERACTIONS: “Tools” for Understanding the Molecular, Cellular, and Physiological, Bases of the Tissue Response to Implants M. Spector, Ph.D. and I.V. M. Spector, Ph.D. and I.V. Yannas, Ph.D. Massachusetts Institute of Technology Harvard Medical School Brigham and Women’s Hospital VA Boston Healthcare System BIOMATERIALS BIOMATERIALS-TISSUE INTERACTIONS INTERACTIONS Tissue* + Biomaterial** Tissue* + Biomaterial** Cell+Matrix** Matrix** * Structure comprising cells of the same type ** Solid surface Page 1
CELL-MATRIX INTERACTIONS In Tissue Cell Extracellular Matrix In Tissue Engineering Scaffolds Cell Biomaterial Scaffold CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS Control volume Unit cell Processes Types of Ti Issues Tissue formation and remodeling in vitro Wound healing in vivo Page 2
In Tissue In Tissue Cell + Extracellular Extracellular Matrix In Tissue Engineering Scaffolds In Tissue Engineering Scaffolds Cell + Biomaterial Scaffold Cell + Biomaterial Scaffold CELL-MATRIX INTERACTIONS MATRIX INTERACTIONS CONCEPTS FOR UNDERSTANDING CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS • Control Volume • Unit Cell Processes • Types of Tissues • Tissue Formation and Remodeling Tissue Formation and Remodeling In Vitro • Wound Healing Wound Healing In Vivo Page 2
Articular Cartilage A Cell Chondrocytes(P2 Canine)in a Type I Collagen-GAGScaffold Image removed due to copyright considerations. “ Control volun Source:B. Kinner Page 3
2 Cell Matrix Extracellular Articular Cartilage 5 3 1 4 6 40min Chondrocytes (P2 Canine) in a . Source: B. Kinner Type I Collagen-GAG Scaffold B. Kinner “Control Volume” Image removed due to copyright considerations Page 3
UNIT CELL PROCESSES Concept of a“ Control Volume” around a Cell Soluble Regulator Cell+ Matrix Product+Soluble (Regulator) Regulator b Control volume UNIT CELL PROCESSES Concept of a“ Control volume” around a cel Soluble Regulator Mechanical oading(Strain) Cell Matrix Product+ Soluble (Regulator) Regulator B “ Control volume” Page 4
UNIT CELL PROCESSES Concept of a “Control Volume” around a Cell Cell + Matrix Product Product + Soluble (Regulator) (Regulator) Regulator B “Control Volume” Soluble Regulator A UNIT CELL PROCESSES Concept of a “Control Volume” around a Cell Cell + Matrix Product Product + Soluble (Regulator) (Regulator) Regulator B “Control Volume” Soluble Regulator A Mechanical Loading (Strain) Page 4
CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS Control volume Unit cell processes Types of Tissues Tissue formation and remodeling n vitro Wound Healing In vivo UNIT CELL PROCESSES Mitosis Migration Synthesis Contraction Endocytosis Exocytosis Page 5
CONCEPTS FOR UNDERSTANDING CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS • Control Volume • Unit Cell Processes • Types of Tissues • Tissue Formation and Remodeling Tissue Formation and Remodeling In Vitro • Wound Healing Wound Healing In Vivo UNIT CELL PROCESSES UNIT CELL PROCESSES • Mitosis • Migration • Synthesis • Contraction • Endocytosis • Exocytosis Page 5