CELL-MATRIX INTERACTIONS WITH COLLAGEN-GAG MATRICES IN VITRO Cell interactions with the scaffold Mitosis, migration, synthesis, and contraction, and their interrelationships Can provide insights into scaffold composition and structure for improved performance in regenerative medicine Can provide insights into cell behavior
CELL –MATRIX INTERACTIONS WITH MATRIX INTERACTIONS WITH COLLAGEN COLLAGEN-GAG MATRICES GAG MATRICES IN VITRO IN VITRO • Cell interactions with the scaffold Cell interactions with the scaffold – –Mitosis, migration, synthesis, and Mitosis, migration, synthesis, and contraction, and their interrelationships contraction, and their interrelationships • Can provide insights into scaffold Can provide insights into scaffold composition and structure for improved composition and structure for improved performance in regenerative medicine performance in regenerative medicine • Can provide insights into cell behavior Can provide insights into cell behavior in vivo in vivo
Chondrocytes(Passage 2 Canine) in a Type I Collagen-GAG Matrix Live cell imaging for a period of 5 hours Image removed due to copyright considerations J. Cheng
Chondrocytes (Passage 2 Canine) in a Type I Collagen-GAG Matrix Live cell imaging for a period of 5 hours. Image removed due to copyright consi derati ons. J. Cheng
CELL-MATRIX INTERACTIONS Mitosis Migration ynthesis Contraction
CELL –MATRIX INTERACTIONS MATRIX INTERACTIONS • Mitosis Mitosis • Migration Migration • Synthesis Synthesis • Contraction Contraction