Hormon Receptor CH2OH Adenylyl cyclas HO-C-H Adipocyte CH2OH CAMP ATP glycerol ATP kinase,ADP Fatty acids CH,OH HO H L- Glycerol CH。0-P-O 3-phosphate NADT Glycerol glycerol-3-phosphate dehydrogenase NADH +H CHOH O=C Dihydroxyacetone CH2-0-P-0 phosphate Bloodstream triose phosphate H O ATP cO, H-C-OH O D-Glyceraldehyde B oxid 3-phospha citric acid cycle, CH2-0-P-0 Fatty acid respiratory chain transporter Myocyte(muscle) Glycolysis
Glycolysis
4. Early labeling experiments (1904) fatty acids are degraded by sequential removal of two-carbon units When dogs were fed with odd-numbered fatty acids attached to a phenyl group, benzoate was excreted and when fed with even-numbered, phenylacetate was excreted Hypothesis: the p-carbon is oxidized, with two carbon units released by each round of oxidation These experiments are a landmark in biochemistry in using synthetic label(the phenyl group here)to elucidate reaction mechanism, and was done long before radioisotopes was used in biochemistry
4. Early labeling experiments (1904): fatty acids are degraded by sequential removal of two-carbon units • When dogs were fed with odd-numbered fatty acids attached to a phenyl group, benzoate was excreted; and when fed with even-numbered, phenylacetate was excreted. • Hypothesis: the b-carbon is oxidized,with twocarbon units released by each round of oxidation. • These experiments are a landmark in biochemistry, in using synthetic label (the phenyl group here) to elucidate reaction mechanism, and was done long before radioisotopes was used in biochemistry!
CH<CH,-C Franz Knoop's labeling Phenylpropionate (Fatty acid with odd number of carbon atoms Experiments(1904) fatty acids are degraded C by oxidation at theβ Benzoate carbon. 1.e oxidation CH2-CH2+CH2-C Phenylbutyrate Fatty acid with even number of carbon atoms) CH2C、+C2 Phenylacetate
Franz Knoop’s labeling Experiments (1904): fatty acids are degraded by oxidation at the b carbon, i.e., b oxidation . b ba a
5. Fatty acid oxidation was found to occur in mitochondria Enzymes of fatty acid oxidation in animal cells were localized in the mitochondria matrix Revealed by eugene Kennedy and Albert lehninger n1948
5. Fatty acid oxidation was found to occur in mitochondria • Enzymes of fatty acid oxidation in animal cells were localized in the mitochondria matrix. • Revealed by Eugene Kennedy and Albert Lehninger in 1948
家 Albert L. Lehninger 1917-1986