科命生学Anionorcationexchanger贵ifsamplecomponentsaremoststablebelowtheirpl,useacationexchanger个if samplecomponentsaremoststableabovetheirpl,useananionexchanger.If stabilityishighoverawidepHrangeonbothsidesofthepl,useeithertypeofionexchanger.RangeofstabilityoaAttachedtoAnionanionexchangers2exchanger09AttachedtocationexchangersFig2.3.ConsiderationswhenselectingasuitablelEXmedium唐门大了上干至美强王肉
自强不息 止于至善 Anion or cation exchanger Anion exchanger
Test tube method to determine the initial pH1.put1ml anionexchangerinto several tubes2.add buffers with different pH's3.add sample,mix4.analyse the supernatants for the protein ofinterest6.06.57.07.58.0Here the target binds completely at pH 7.5 andpHaboveConclusion:anion exchanger,initialpH7.5Target Protein pl?6.5-7.0厦门大王强不止于至善
自强不息 止于至善 1.put 1 ml anion exchanger into several tubes 2.add buffers with different pH's 3.add sample, mix 4.analyse the supernatants for the protein of interest Here the target binds completely at pH 7.5 and above 6.0 6.5 7.0 7.5 8.0 pH Test tube method to determine the initial pH Target Protein pI? 6.5-7.0 Conclusion: anion exchanger, initial pH 7.5
Fig1.14.Structureof cross-linkedagarosemedia (Sepharose)门大了止于至姜福工电
自强不息 止于至善