Whathappens inionexchange?regenerationimaginationatwork
- - - - - - - - - - - - - - - - - - - - - - - + + + + + + regeneration What happens in ion exchange?
科命生2AeeSCIENCESXIAKey:o Large net positive charge Net positive chargeONet negative chargeLarge net negative chargePolymer beadswith negativelychargedfunctional groupsProtein mixture is addedto column containingcation exchangers.Proteins move through thecolumn at rates determinedby their net charge at thepli being used. With cationexchangers, proteins withamorenegativenetcharge15fmove faster and elute earlier.(a)唐门大王止干至美
自强不息 止于至善
科命生C离子交换剂的类型：阳离子交换剂Cationexchanger（带负电，吸附带正电的蛋白质）CM(羧甲基)-纤维素或琼脂糖 ，SP，S阴离子交换剂Anionexchanger（带正电，吸附带负电的蛋白质）：DEAE(二乙氨基乙基)-纤维素或琼脂糖，QAE，Q门大王强不鸟止干至美
自强不息 止于至善 离子交换剂的类型： 阳离子交换剂Cation exchanger（带负电，吸附带正电的蛋白质）： CM(羧甲基)-纤维素或琼脂糖 ，SP，S 阴离子交换剂Anion exchanger（带正电，吸附带负电的蛋白质） ： DEAE(二乙氨基乙基)-纤维素或琼脂糖 ， QAE，Q
科命生学米Table1.2.Functional groups used on ion exchangersFunctional groupAnionexchangersstrong-CH,-N*-(CH,)sOuaternaryammoniumO)weakDiethylaminoethyl (DEAE)*-CH,-CH,-N*-(CH,-CH,)2weakDiethylaminopropyl(ANX)*-CH,-CHOH-CH,-N+-(CH,-CH,)2CationexchangersFunctional groupSulfopropyl(SP)strong-CH,-CH,-CH,-SO,-CH,-SO;Methyl sulfonate(S)strongweakCarboxymethyl(CM)-CH,-COO-唐门大了止干至姜强王肉
自强不息 止于至善
命生CSelectivityandbufferpHCationexchanger:Most acidic pHAbsAbsAbsAbsMIMLACation+pHAnionAbsAbsAnionAAAexchanger:LessalkalipH唐门大王止干至美强工电
自强不息 止于至善 Selectivity and buffer pH Cation exchanger： Most acidic pH Anion exchanger： Less alkali pH