上游文通大学 SHANGHAI JIAO TONG UNIVERSITY INTRODUCTION Protein (macromolecule,amino acid,peptide, structure,configuration,function) genome How to Explore? chromosomes .-----genes Genes contain instructions for making proteins Proteins act alone or in complexes to perform many cellular functions
Protein (macromolecule，amino acid，peptide， structure，configuration，function) INTRODUCTION How to Explore？
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY INTRODUCTION Development: chip-based mass spectrometry approaches complex mixture analysis LC-MS(/MS) Proteins 2D gel electrophoresis proteomics 一time 1975 1990 1994 1996 2002 algorithms nucleotide sequencing Proteomes ESTs/genome scale genetic approaches
INTRODUCTION Development： Proteins Proteomes
上游充通大 OUTLINES SHANGHAI JIAO TONG UNIVERSITY Proteins 1.The Purification of Proteins Is an Essential First Step in Understanding Their Function 2.The Characterization of Proteins:Molecular Weight,isoelectric point. 3.Amino Acid Sequencing and primary structure
1.The Purification of Proteins Is an Essential First Step in Understanding Their Function 2. The Characterization of Proteins: Molecular Weight, isoelectric point. 3 . Amino Acid Sequencing and primary structure OUTLINES Proteins
上游充通大兽 OUTLINES SHANGHAI JIAO TONG UNIVERSITY Proteomes 4.Two-dimensional electrophoresis is currently the workhorse for the majority of ongoing proteome projects. 5.Mass Spectrometry Provides Powerful Tools for Protein Characterization and Identification 6.Three-Dimensional Protein Structures Can Be Determined by X-ray Crystallography and NMR Spectroscopy
4.Two-dimensional electrophoresis is currently the workhorse for the majority of ongoing proteome projects. 5. Mass Spectrometry Provides Powerful Tools for Protein Characterization and Identification 6.Three-Dimensional Protein Structures Can Be Determined by X-ray Crystallography and NMR Spectroscopy OUTLINES Proteomes
上游充通大学 OUTLINES SHANGHAI JIAO TONG UNIVERSITY Proteins 1.The Purification of Proteins Is an Essential First Step in Understanding Their Function 2.The Characterization of Proteins:Molecular Weight,isoelectric point. 3.Amino Acid Sequencing and primary structure
OUTLINES Proteins 1.The Purification of Proteins Is an Essential First Step in Understanding Their Function 2. The Characterization of Proteins: Molecular Weight, isoelectric point. 3 . Amino Acid Sequencing and primary structure