Extraction of Vitamin A 1.Weigh 5-10g of the previously crushed food substance into a 1 L round flask. 2.Add 20 ml of a 50%NaOH solution and warm the mixture in a water bath. 3.Then,add 100 ml of diethyl alcohol and 2 ml of a hydroquinone solution that was obtained by dissolving 20 g in 100 ml of pure alcohol. 4.Maintain the water bath at 90'C for 30 minutes. 5.Pour the contents of the round flask into a decanting vial and add 100 ml of water. 6.Add 50 ml of ethylic ether and shake. 7.Add 50 ml of petroleumether.Shake and allow it to decant. 8.Extract once or twice with 50 ml of petrol ether. 9.Wash the ether phase three times with 100 ml of water. 10.Filter,evaporate,and concentrate until 1 ml is obtained. It must be noted that all of these steps are conducted away from light Moreover,saponification with NaOH is not useful with nonfatty produds
1. Weigh 5-10 g of the previously crushed food substance into a 1 L round flask. 2. Add 20 ml of a 50% NaOH solution and warm the mixture in a water bath. 3. Then, add 100 ml of diethyl alcohol and 2 ml of a hydroquinone solution that was obtained by dissolving 20 g in 100 ml of pure alcohol. 4. Maintain the water bath at 90℃ for 30 minutes. 5. Pour the contents of the round flask into a decanting vial and add 100 ml of water. 6. Add 50 ml of ethylic ether and shake. 7. Add 50 ml of petroleum ether. Shake and allow it to decant. 8. Extract once or twice with 50 ml of petrol ether. 9. Wash the ether phase three times with 100 ml of water. 10. Filter, evaporate, and concentrate until 1 ml is obtained. It must be noted that all of these steps are conducted away from light. Moreover, saponification with NaOH is not useful with nonfatty products. Extraction of Vitamin A
Extraction of Vitamin D2 or D3 1.Weigh between 5 and 10 g of the sample food substance. 2.Add 1 g of pyropanol,90 ml of a mixture of 60 ml absolute ethanol,and 30 ml of a 50%potash solution. 3.Extract three times,each time with 50 ml of petroleum ether. 4.Wash the ether extract and material three times with water. 5.Filter,evaporate,and concentrate until 1 ml is obtained. Saponification with the alcoholic potash mixture is not necessary if the products to be analyzed do not contain fats
1. Weigh between 5 and 10 g of the sample food substance. 2. Add 1 g of pyropanol, 90 ml of a mixture of 60 ml absolute ethanol, and 30 ml of a 50% potash solution. 3. Extract three times, each time with 50 ml of petroleum ether. 4. Wash the ether extract and material three times with water. 5. Filter, evaporate, and concentrate until 1 ml is obtained. Saponification with the alcoholic potash mixture is not necessary if the products to be analyzed do not contain fats. Extraction of Vitamin D2 or D3