Flowsheet for developing an industrial microbial fermentation process Strain selection Laboratory process development Pilot Scale up Industrial Scale up Downstream process development Product packaging techniques Other commercial consideration Examples
Flowsheet for developing an industrial microbial fermentation process • Strain selection • Laboratory process development • Pilot Scale up • Industrial Scale up • Downstream process development • Product packaging techniques • Other commercial consideration • Examples
Downstream Process Prehminary treatments Development Ferment Solids Filtration Filter(press filter) Liquid Alternate purification method Extract Drac Adsorption Chemical Solvent columns precipitaton extraction Wast Chromatography Partially purified antibiotic in solution Recrystallization Dryer Purification Animal feed supplement Antibio
Downstream Process Development Chromatography Filter (press filter)
Downstream Process Development Stock culture Flask culture Seed culture Sterile medum Intermediate Antifoam fermentor Continuous centrifuges Feist centration washing °g° Cool storage Production fermentor () Dewatering→+ Compressing Packaging--Cold storageCompressed yeast cakes ing--Extruding→d→ Packaging-- Cool storag Active dry yeast Pasteurizing→ Drum drying→ Grinding→- Packaging→ Dry storage Nutritional dry yeast
Downstream Process Development Continuous centrifuges
Unit Operations in Downstream Separation · Microfilters · Ultrafiltration Size Gel chromatograp Reverse osmosis Diffusivity Dialysis Electrodialysis Ion exchange on exchange Distillation/freeze dr Vapour temperature, pressure · Solvent extraction Solubility Foam and bubble fractionation Surface activity Ultracentrifugation ° Centrifugation Density Liquid cyclones Gravity sedimentation
Unit Operations in Downstream Separation • Microfilters • Ultrafiltration • Gel chromatography • Reverse osmosis • Dialysis • Electrodialysis • Ion exchange • Distillation/freeze dry • Solvent extraction • Foam and bubble fractionation • Ultracentrifugation • Centrifugation • Liquid cyclones • Gravity sedimentation Size Diffusivity Ion exchange Vapour temperature, pressure Solubility Surface activity Density
Processing fermentation broth Fermentation Dilute slurry Centrifugation filtration Sedimentation Clarified liquor Concentrated slur Filtration Dewatering Drying Bright liquor Dry biomass
Processing fermentation broth Fermentation Dilute slurry Centrifugation Filtration Sedimentation Clarified liquor Filtration Bright liquor Concentrated slurry Dewatering Drying Dry Biomass