Growth Culture of bacteria Chapter 4, 5
Growth & Culture of Bacteria Chapter 4, 5
Key Words Obligate aerobe Obligate anaerobe Generation time Facultative anaerobe Microaerophilic Growth curve Mesophile Thermophile Psychrophile
Key Words Obligate aerobe Obligate anaerobe Facultative anaerobe Microaerophilic Mesophile Thermophile Psychrophile Generation time Growth curve 2
Growth of bacteria Bacterial Multiplication- Binary Division 1 to 2 to 4 to 8 to n Cell wal Cell membrane Log Growth Elongated nucleoid Nucleoid divides: cell wall and membrane Number begin to form transverse septum 20 Transverse septum becomes complete Daughter cells Chapter 4 Generations separate
Growth of bacteria Bacterial Multiplication - Binary Division 1 to 2 to 4 to 8 to N Log Growth ? Chapter 4
Stationary phase Death or Log or logarithmic exponential decline phase a growth phase Lag hase Chapter 4 Fig. 4-2 Time Source: Brooks GF, Carroll KC, Butel. Phase Medical Microbiology, 26th Edition: w Growth rate Lag Zero Copyright o The McGraw-Hill Compal Exponential Constant Maximum stationary zero Decline Negative(death)
Phase Growth Rate Lag Zero Exponential Constant Maximum stationary Zero Decline Negative (death) Chapter 4 Fig. 4-2
Phases of bacteria growth(in vitro) Growth curve Lag Adapt to nutrients Log Active growth Stationary Death Growth rate Death Nutrients consumed pH too low(why? Optimize curves in production
Phases of bacteria growth (in vitro) Growth curve • Lag – Adapt to nutrients • Log – Active growth • Stationary – Death = Growth rate • Death – Nutrients consumed – pH too low (why?) • Optimize curves in production