Plasmid transformation Zhang Junqi, Han Wendong 2012-10-11
1 Plasmid Transformation Zhang Junqi, Han Wendong 2012-10-11
1. Experiments: Transformation of plasmid dna 2. Demonstration Plasmid extraction
2 1. Experiments: Transformation of Plasmid DNA 2. Demonstration: Plasmid extraction
Characteristics of plasmids Plasmid is a type of dna existence within bacteria >extrachromosomal genomes >self-replicating >commonly used vectors
3 Characteristics of plasmids Plasmid is a type of DNA existence within bacteria extrachromosomal genomes self-replicating commonly used vectors
Some bacterial characteristics are determined by plasmids L Antibiotic resistance Many bacteria can become resistant to antibiotics by acquisition of a plasmid 2. Colicins and bacteriocins Another property conferred by some plasmids that has been widely studied is the ability to produce a protein which has an antimicrobial action 3. Virulence determinants In some bacterial species toxin genes are carried on plasmids
4 Some bacterial characteristics are determined by plasmids 1.Antibiotic resistance Many bacteria can become resistant to antibiotics by acquisition of a plasmid 2.Colicins and bacteriocins Another property conferred by some plasmids that has been widely studied is the ability to produce a protein which has an antimicrobial action 3.Virulence determinants In some bacterial species toxin genes are carried on plasmids
Nucleoid Plasmids Plasmids are small circular DNA molecules that are found inside some prokaryotic cells schematic diagram of plasmid
5 schematic diagram of plasmid