Experiment 2&3 1. Video: Culture Media Preparation and autoclaving 2. Discussion: Bacterial culture Sterilization and disinfection 3. Exercises. All students: Streak plates Group 1: Examination of the bacterial distribution Group 2: The ultraviolet radiation test Group 3: Effectiveness of hand washing
Experiment 2 & 3 1. Video: 2. Discussion: 3. Exercises: Culture Media Preparation and Autoclaving Bacterial culture Sterilization and disinfection All students: Streak plates Group 1: Examination of the bacterial distribution Group 2: The ultraviolet radiation test Group 3: Effectiveness of hand washing
Experiment 2 Bacterial cultivation
Experiment 2 Bacterial Cultivation
Requirements for bacterial growth Nutrients H2O, C-source, N-source, Inorganic salts, Growth factors Temperature ph Gas incubator Temperature, gas culture medium (R Nutrients, pH
3 Requirementsfor bacterial growth • • • Temperature Nutrients H2O, C-source, N-source, Inorganic salts, Growth factors pH Gas Temperature, gas Nutrients, pH n incubator culture medium ® ®
Culture medium is the mixture of various nutrients that is suitable for the growth of microorganisms Video: Culture medium preparation and autoclaving
4 n Culture medium – is the mixture of various nutrients that is suitable for the growth of microorganisms. Culture medium preparation and autoclaving Video:
Types of Culture Media based on the physical state based on the function and chemical components
n Types of Culture Media • • based on the physical state based on the function and chemical components