Experiment IV Microbiological examination of bacteria Pan Tingting, PhD Key lab of Medical Molecular virology, Shanghai Medical College, Fudan University
Experiment Microbiological examination of bacteria IV Pan Tingting, PhD Key Lab of Medical Molecular Virology, Shanghai Medical College, Fudan University
Contents 1. Video Lab diagnosis of bacterial infection 2. Discussion Diagnostic principle of bacterial infection b) How to write a recorder of an experiment 3. Operation a) isolate bacterium from stool(lst): inoculation onto Mac con key agar b) Acid-fast stain 4. Design of widal test 5. Demonstration Mycobacterium and its colonies
Contents 1. Video Lab diagnosis of bacterial infection 2. Discussion a) Diagnostic principle of bacterial infection b) How to write a recorder of an experiment 3. Operation a) isolate bacterium from stool(1 st ): inoculation onto MacConkey agar b) Acid-fast stain 4. Design of widal test 5. Demonstration Mycobacterium and its colonies ： ： ： ：
Diagnosis of bacterial Infection Patient Clinical Non-microbiological diagnosis investigations Radiology Haematology Biochemistry Sample Take the correct specimen Take the specimen correctly Label package the specimen up correctly Appropriate transport storage of specimen A proper clinical assessment is essential for optimal use of laboratory services!
Diagnosis of Bacterial Infection Patient Clinical diagnosis Non-microbiological investigations Sample Take the correct specimen Take the specimen correctly Label & package the specimen up correctly Appropriate transport & storage of specimen A proper clinical assessment is essential for optimal use of laboratory services! Haematology Biochemistry Radiology
Principle of collection asepsIs avoid contamination by normal flora or bacteria from environment 2. take the correct specimen specimens include blood, urine, pus, stool, sputum, CSF, or other material, as indicated by the localization of the disease process 3. early before using antibiotics 4. fresh test ASAP (As Soon as possible
Principle of collection 1． 2. 3． ． asepsis avoid contamination by normal flora or bacteria from environment. take the correct specimen specimens include blood, urine, pus, stool, sputum, CSF, or other material, as indicated by the localization of the disease process. early before using antibiotics 4 fresh test ASAP（As Soon As Possible ）
Disease or symptoms specimens septicemia Blood specimen espiratory tract infection Sputum specimen urinary infection mid-stream urine digestive tract infection vomitus or stool purulent infection us secreta form STD Urogenital Tract epidemic meningitis CSF or blood enteric fever Blood(1-2weeks) Stool (2-3weeks
septicemia epidemic meningitis enteric fever Blood specimen Disease or symptoms specimens respiratory tract infection Sputum specimen urinary infection mid-stream urine digestive tract infection vomitus or stool purulent infection pus STD secreta form Urogenital Tract CSF or blood Blood (1-2weeks) Stool (2-3weeks)