Experiment 5 Jungi zhang Department of Medical Microbiology Parasitology Shanghai Medical College, Fudan University
Experiment 5 Junqi Zhang Department of Medical Microbiology & Parasitology Shanghai Medical College, Fudan University
Experiment 5 1. Widal test 2. Ziehl Neelsen Stain(Acid-Fast stain) 3. Identification of Predominant isolates in the Gastrointestinal tract Gram stain Broth inoculation 10/25/2012 Experiment 5/ Junqi Zhang
10/25/2012 Experiment 5/ Junqi Zhang 2 Experiment 5 1.Widal test 2.Ziehl Neelsen Stain (Acid-Fast stain) 3.Identification of Predominant isolates in the Gastrointestinal Tract -Gram Stain -Broth Inoculation
Widal test Patientssuffering from enteric fever would possess antibodies(agglutinins) in their sera Widal test is a tube agglutination test for the detection of agglutinins(antibodies) for H and O antigen for salmonella in patients with enteric fever The test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist, born March 9, 1862, Algeria; died January14, 1929, Paris The result will be used as an indirect signal bf the infection
Patients’ suffering from enteric fever would possess antibodies (agglutinins) in their sera Widal test is a tube agglutination test for the detection of agglutinins (antibodies) for H and O antigen for salmonella in patients with enteric fever The test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist, born March 9, 1862, Algeria; died January14, 1929, Paris The result will be used as an indirect signal of the infection Widal test
3. Widal test: Tube dilution agglutination Test Principle Patients' suffering from enteric fever would possess antibodies in their sera which can react and agglutinate serial doubling dilutions of killed, coloured Salmonella antigens in a tube agglutination test Widal test is named after georges fernand isidore widal a French physician and bacteriologist, born March 9, 1862 Algeria; died January 14, 1929, Paris
4 3.Widal test: Tube Dilution Agglutination Test Patients’ suffering from enteric fever would possess antibodies in their sera which can react and agglutinate serial doubling dilutions of killed, coloured Salmonella antigens in a tube agglutination test Principle: Widal test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist, born March 9, 1862, Algeria; died January14, 1929, Paris
Widal test Serum agglutinins(antibodies) rise sharply during the second and third weeks of salmonella typhi infection This test demonstrates the presence of somatic(O)and flagellar(H)agglutinins(antibodies) to Salmonella typhi in the patient's serum using suspensions ofO and H antigens Antigens of S paratyphi A, S paratyphi b,s paratyphi C are included in most commercial kits The result will be used as an indirect signal of the infection
5 Serum agglutinins(antibodies) rise sharply during the second and third weeks of Salmonella Typhi infection This test demonstrates the presence of somatic (O) and flagellar (H) agglutinins(antibodies) to Salmonella typhi in the patient's serum using suspensions of O and H antigens. Antigens of S. paratyphi A, S. paratyphi B, S. paratyphi C are included in most commercial kits The result will be used as an indirect signal of the infection Widal test