Note the drop-off as you get closer to the Optimum! Enzymes and reactions occurring at maximal possible rate Enzymes and reactions Optimum occurring at increasingly rapid rates Minimum Maximum Temperature Membrane gelling; transport Protein denaturation; collapse processes so slow that of the cytoplasmic membrane; growth cannot occur thermal lysis
Note the drop-off as you get closer to the Optimum!
Bacterial Growth Curve Growth phases E0E Lag Exponential s Stationary Death 9.0 110 Turbidity 10.75 8.0 Viable count (optical density) 050 7.0 8 025 ⊙6.0 50 0.1 Time Is it the same as in human body Why?
Is it the same as in human body ? Why? Bacterial Growth Curve
Nutritional Factors Carbon Source Hetero- or auto- Organic compounds or cO2 Nitrogen Source NO3==> NO2==>NH3 to Amino Acids==> Proteins Sulfur ° Phosphorus Trace Elements Fe, some required for growth or virulent Host sideophores bind Fe
Nutritional Factors • Carbon Source – Hetero- or Auto- – Organic compounds or CO2 • Nitrogen Source – NO3- ==> NO2 ==> NH3 to Amino Acids ==> Proteins • Sulfur • Phosphorus • Trace Elements – Fe, some required for growth or virulent – Host sideophores bind Fe