COLLAGEN-GAG MATRICES: MODEL BIOMATERIALS (ANALOGS OF EXTRACELLULAR MATRIX) Investigation of cell interactions(UCPs)in vitro Type I (bovine and porcine) ype II( e) Chondroitin 6-sulfate copyright considerations Image removed due to opyright considerations Imm · Freeze- dried Dehydrothermally cross-linked Additional cross-linking 500um CELL-MATRIX INTERACTIONS WITH COLLAGEN-GAG MATRICES IN VITRO an provide insights into interrelationships among cell processes How do mitosis and synthesis interrelate? How do mitosis and synthesis relate to contraction? How does migration relate to contraction? an provide insights into cell behavior in vivo Can provide insights into scaffold composition and structure for improved performance in regenerative medicine Page 6
1mm 500mm COLLAGEN-GAG MATRICES: MODEL BIOMATERIALS (ANALOGS OF EXTRACELLULAR MATRIX) Investigation of cell interactions (UCPs ) in vitro • Type I (bovine and porcine) • Type II (porcine) • Chondroitin 6-sulfate • Freeze -dried • Dehydrothermally cross-linked • Additional cross-linking IV Yannas, et al. PNAS, 1989 Image removed due to copyright considerations. Image removed due to copyright considerations. CELL –MATRIX INTERACTIONS WITH MATRIX INTERACTIONS WITH COLLAGEN-GAG MATRICES GAG MATRICES IN VITRO • Can provide insights into interrelationships Can provide insights into interrelationships among cell processes. – How do mitosis and synthesis interrelate? – How do mitosis and synthesis relate to How do mitosis and synthesis relate to contraction? – How does migration relate to contraction? • Can provide insights into cell behavior Can provide insights into cell behavior in vivo. • Can provide insights into scaffold composition Can provide insights into scaffold composition and structure for improved performance in and structure for improved performance in regenerative medicine. Page 6
Chondrocytes(Passage 2 Canine) in a Type I Collagen-GAG Matrix ve cell imaging for a period of 5 hour mage removed due to copyright considerations J. Cheng CELL-MATRIX INTERACTIONS Mitosis Migration Contraction Page 7
Chondrocytes (Passage 2 Canine) in a Type I Collagen-GAG Matrix J. Cheng Live cell imaging for a period of 5 hours. Image removed due to copyright considerations. CELL–MATRIX INTERACTIONS MATRIX INTERACTIONS • Mitosis • Migration • Synthesis • Contraction Page 7
Chondrocyte(P2 Canine) in a Type I Collagen GAG matrix Mitosis J. Cheng Effects of Cross-Linking on Chondrocyte Proliferation in Collagen-GAG Matrices Mean±SEM - DHT GTA EDAC 0 10 20 30 Day Page 8
DNA Content Chondrocyte (P2 Canine) in a Type I Collagen-GAG Matrix: Mitosis J. Cheng 0 10 20 30 40 50 60 0 10 20 30 Day DNA (ug) DHT UV GTA EDAC CR Lee, et al., Biomat. 2001;22:3145 Mean – SEM Effects of Cross-Linking on Chondrocyte Proliferation Linking on Chondrocyte Proliferation in Collagen-GAG Matrices Page 8