LIQUID-LIQUID CHROMATOGRAPHY ODPN(oxydipropionylnitrile) Normal Phase LLC Reverse Phase LLC NCCH 3 CH 2 OCH 2 CH 2 CN(Normal) CH 3 (CH 2 )16 CH 3 (Reverse) The stationary solid surface is coated with a 2nd liquid (the Stationary Phase) which is immiscible in the solvent (Mobile) phase. Partitioning of the sample between 2 phases delays or retains some components more than others to effect separation
LIQUID-LIQUID CHROMATOGRAPHY ODPN(oxydipropionylnitrile) Normal Phase LLC Reverse Phase LLC NCCH 3 CH 2 OCH 2 CH 2 CN(Normal) CH 3 (CH 2 )16 CH 3 (Reverse) The stationary solid surface is coated with a 2nd liquid (the Stationary Phase) which is immiscible in the solvent (Mobile) phase. Partitioning of the sample between 2 phases delays or retains some components more than others to effect separation
M O B IL E P H A S E L IQ U ID L iq u id -L iq u id C h r o m a to g r a p h y (P a rtitio n ) L iq u id -S o lid C h r o m a to g r a p h y (A d s o r p tio n ) L iq u id S o lid N o rm a l P h a s e R e v e rs e P h a s e N o rm a l P h a s e R e v e rs e P h a s e M o b il e P h a s e - N o n p o l a r S t a tio n a r y p h a s e - P o l a r M o b il e P h a s e - P o l a r S t a tio n a r y p h a s e - N o n p o l a r F O R M A T S T A T IO N A R Y P H A S E
M O B IL E P H A S E L IQ U ID L iq u id -L iq u id C h r o m a to g r a p h y (P a rtitio n ) L iq u id -S o lid C h r o m a to g r a p h y (A d s o r p tio n ) L iq u id S o lid N o rm a l P h a s e R e v e rs e P h a s e N o rm a l P h a s e R e v e rs e P h a s e M o b il e P h a s e - N o n p o l a r S t a tio n a r y p h a s e - P o l a r M o b il e P h a s e - P o l a r S t a tio n a r y p h a s e - N o n p o l a r F O R M A T S T A T IO N A R Y P H A S E