VITAMINS Vitamin A H3 C CH3 CH3 CH3 CH3 CH2 O H
VITAMINS Vitamin A H3 C CH3 CH3 CH3 CH3 CH2 O H
b - Carotene CH3 CH3 CH3 CH3 CH3 CH3 CH3 CH3 H3C CH3 H3C CH3 CH3 CH3 CH3 H3C CH3 CH3 CH3 CH3 CH2OH Oxidation C H O Retainal Retinol (Vitamin A) - 2H
b - Carotene CH3 CH3 CH3 CH3 CH3 CH3 CH3 CH3 H3C CH3 H3C CH3 CH3 CH3 CH3 H3C CH3 CH3 CH3 CH3 CH2OH Oxidation C H O Retainal Retinol (Vitamin A) - 2H
Food KOH (Alcoholic) Saponification 3 hrs. at room temperature Ether for Extraction Extract (vit.A and carotenoids) Total Carotenoids only at 440 nm Vitamin A + Carotenes Carr-Price Reagent Measure at 620 nm Vitamin A and b - Carotene Determination
Food KOH (Alcoholic) Saponification 3 hrs. at room temperature Ether for Extraction Extract (vit.A and carotenoids) Total Carotenoids only at 440 nm Vitamin A + Carotenes Carr-Price Reagent Measure at 620 nm Vitamin A and b - Carotene Determination
b-CAROTENE STANDARD ABSORBANCES AT 440 AND 620 nm at 440 nm at 620 nm A A
b-CAROTENE STANDARD ABSORBANCES AT 440 AND 620 nm at 440 nm at 620 nm A A
Use absorbances at 440 nm and then convert this to absorbance at 620 nm and subtract from the absorbance at 620 nm to determine the absorbance at 620 due to Vitamin A. Carotenoid Absorbance at 440nm Vitamin A Absorbance at 620 nm Carotenoid (mg/ml) Vitamin A mg/cuvette (sample) x x x x x x x x x x x
Use absorbances at 440 nm and then convert this to absorbance at 620 nm and subtract from the absorbance at 620 nm to determine the absorbance at 620 due to Vitamin A. Carotenoid Absorbance at 440nm Vitamin A Absorbance at 620 nm Carotenoid (mg/ml) Vitamin A mg/cuvette (sample) x x x x x x x x x x x