The 4 basic liquid chromatography modes are named according to the mechanism involved: 1. Liquid/Solid Chromatography (adsorption chromatography) A. Normal Phase LSC B. Reverse Phase LSC 2. Liquid/Liquid Chromatography (partition chromatography) A. Normal Phase LLC B. Reverse Phase LLC 3. Ion Exchange Chromatography 4. Gel Permeation Chromatography (exclusion chromatography) FOUR BASIC LIQUID CHROMATOGRAPHY
The 4 basic liquid chromatography modes are named according to the mechanism involved: 1. Liquid/Solid Chromatography (adsorption chromatography) A. Normal Phase LSC B. Reverse Phase LSC 2. Liquid/Liquid Chromatography (partition chromatography) A. Normal Phase LLC B. Reverse Phase LLC 3. Ion Exchange Chromatography 4. Gel Permeation Chromatography (exclusion chromatography) FOUR BASIC LIQUID CHROMATOGRAPHY
LIQUID SOLID CHROMATOGRAPHY Si - O - H Normal phase LS Reverse phase LS Silica Gel The separation mechanism in LSC is based on the competition of the components of the mixture sample for the active sites on an absorbent such as Silica Gel
LIQUID SOLID CHROMATOGRAPHY Si - O - H Normal phase LS Reverse phase LS Silica Gel The separation mechanism in LSC is based on the competition of the components of the mixture sample for the active sites on an absorbent such as Silica Gel
LIQUID SOLID CHROMATOGRAPHY Si - OH HEXANE OH C-CH3 CH3 CH3- C CH3 CH3 OH OH CH3 CH3
LIQUID SOLID CHROMATOGRAPHY Si - OH HEXANE OH C-CH3 CH3 CH3- C CH3 CH3 OH OH CH3 CH3
WATER-SOLUBLE VITAMINS 1. Niacinamide 2. Pyridoxine N CONH2 N CH2OH CH2OH HO H3C 3. Riboflavin N N NH N CH2 HOCH HOCH HOCH CH2OH O H3C O H3C Cl N S N H3C N CH2 NH2 CH3 CH2CH2OH 4. Thiamin
WATER-SOLUBLE VITAMINS 1. Niacinamide 2. Pyridoxine N CONH2 N CH2OH CH2OH HO H3C 3. Riboflavin N N NH N CH2 HOCH HOCH HOCH CH2OH O H3C O H3C Cl N S N H3C N CH2 NH2 CH3 CH2CH2OH 4. Thiamin
WATER-SOLUBLE VITAMINS 0 5 10 15 20 Column: u Bondapak C18 Solvent: MeOH Sample: Water-Soluble Vitamins Inject 1 2 3 4
WATER-SOLUBLE VITAMINS 0 5 10 15 20 Column: u Bondapak C18 Solvent: MeOH Sample: Water-Soluble Vitamins Inject 1 2 3 4