思考-1如果得不到高质量的晶体？ Published:10 February 2016 Macromolecular diffractive imaging using imperfect crystals Kartik Ayyer Oleksandr M Yefanoy,Dominik Oberthar Shatabdi Roy-Chowdhury,Lorenzo Galli Yalerig Mariani Shibom Basy,Jesse Coe Chelsie F.Conrad Raimund Fromme Aiexander Schaffer Katerina Dorner,Daniel James,Christopher Kupitz,Markus Metz,Garrett Nelson Paulraj Lourdu Xavier Kenneth R. Beyerlein,Marius Schmidt,losifina Sarrou,John C.H.Spence,Uwe Weierstall,Thomas A.White,Jay-How Yang,...Henry N.Chapman +Show authors Noture 530,202-206 (2016)Cite this article 19k Accesses 92 Citations 182 Altmetric Metrics Abstract The three-dimensional structures of macromolecules and their complexes are mainly elucidated by X-ray protein crystallography.A major limitation of this method is access to high-quality crystals,which is necessary to ensure X-ray diffraction extends to sufficiently large scattering angles and hence yields information of sufficiently high resolution with which to solve the crystal structure.The observation that crystals with reduced unit-cell volumes and tighter macromolecular packing often produce higher-resolution Bragg peaksL2 suggests that crystallographic resolution for some macromolecules may be limited not by their heterogeneity,but by a deviation of strict positional ordering of the crystalline lattice. Such displacements of molecules from the ideal lattice give rise to a continuous diffa pattern that is equal to the incoherent sum of diffraction from rigid individual mole 变废为宝Nature,2016,530:202-206
思考-1 如果得不到高质量的晶体？ 变废为宝 Nature，2016，530：202-206 严禁复制
思考-2膜蛋白如何结晶？ 原核、真核约20-30%开 放式阅读框编码膜蛋白 Peripheral Integral Membrane Proteins Membrane Proteins 亚禁复制
思考-2 膜蛋白如何结晶？ 原核、真核约20-30%开 放式阅读框编码膜蛋白 严禁复制
场景-1外周膜蛋白如何提取、结晶？ Peripheral Membrane Protein Extraction 加入选用的Buffer 100,000×g 周边膜蛋白在上清液中 辩育10-30min 低温超高速离心 脂双层与整合膜蛋白等 结柯在沉淀中
场景-1 外周膜蛋白如何提取、结晶？ 严禁复制
场景-2整合膜蛋白如何结晶？ 三传品体类型1 上相分了 模蛋白在天然面 飘近白·去后剂 艰分子层结构中 混合做团复合物 三推品体类型Ⅱ 街类分 去垢剂 抗体片段共结晶 港三米型用
场景-2 整合膜蛋白如何结晶？ 去垢剂 抗体片段共结晶 严禁复制