数据分析小故事 焦急等待的日子 最困难也没放弃…
数据分析小故事 最困难也没放弃...... 焦急等待的日子...... 严禁复制
讨论 思考-1是否可以检测到水分子？ 思考-2是否可以检测到17对二硫键？
讨论 思考-1 是否可以检测到水分子？ 严禁复制 思考-2 是否可以检测到17对二硫键？
提问--根据文中信息进行判断？ Crystallization and X-Ray Data Collection.OsrHSA from large-scale production was solubilized in buffer [50 mM KPO4 (pH 7.5), 150 mM NaCl],and the protein was further purified using size- exclusion chromatography to remove any dimers.The peak corresponding to the monomeric species was collected and the protein was concentrated to 100 mg/mL in 50 mM KPO4 (pH 7.5)and 150 mM NaCl.Results from dynamic light scat- tering showed the presence of only the monomeric species. For preparation of the HSA-myristic acid complex,myristic acid at a concentration of 2.5 mM was resuspended in 20 mM KPO4 (pH 7.5)and warmed to 50 C.The sample was then cooled to ~30 C,and 0.2 mM of rHSA was added.The sample was incubated for 20 min,cooled to 4 C,and centrifuged at 12,000 xg for 4 min at 4 C to pellet any excess myristic acid. The supernatant was concentrated to ~120 mg/mL and buffer exchanged in 20 mM KPO4(pH 7.5)and 0.1 mM myristic acid. Crystals of HSA-myristic acid complex were obtained with the hanging diffusion method in a 28%PEG 3350,50 mM phos- phate buffer(pH 7.5),and 150 mM KCl.The crystals of OsrHSA were flash-frozen in liquid nitrogen,and the X-ray diffraction data were collected at the synchrotron beamline to 2.05 A res- olution with the crystal maintained at the temperature f 100 K. 分辨率
提问 --- 根据文中信息进行判断？ 分辨率 严禁复制
分辨率查询结果 3.0A 5A 大部分主链 Resolution 3.5A 35A 3.5A 主链+大侧链 201 27A 2.5A 主链+小侧链 图1 olution 2.0 A 1.2A 主链+侧链+某些氢原子
分辨率查询结果 5A 大部分主链 3.5A 主链+大侧链 2.5A 主链+小侧链 1.2A 主链+侧链+某些氢原子 严禁复制
为什么？分辨率--最小可观察到的距离 C-C键 C-0键 0-H键 1.5A 1.4-1.5A 0.9A S-C键 S-H键 0-H键 1.7A 1.7A 0.9A
为什么？分辨率 --- 最小可观察到的距离 1.5A 1.4-1.5A 0.9A C-C键 C-O键 O-H键 1.7A 1.7A 0.9A S-C键 严禁复制 S-H键 O-H键