Antiviral therapy- General Principles NIv JIANER LONG. Rh Lab of Medical Microbiology, Department of Medical Microbiology and Parasitology, Shang Medical College of fudan University 138 Yixueyuan R, Shanghai 200032, PR China Email:longjianer@fudan.edu.cn;Tel:+86-21-54237867
Antiviral therapy - General Principles JIANER LONG, Ph.D. Lab of Medical Microbiology, Department of Medical Microbiology and Parasitology, Shang Medical College of Fudan University, 138 Yixueyuan R., Shanghai 200032, PR China Email: longjianer@fudan.edu.cn; Tel.: +86-21-54237867
Effect of chemical& physical agents on viruses Heat is the most reliable method of virus disinfection Most human pathogenic viruses inactivated by exposure to60°cfor30mns Viruses are stable at low temperatures and are routinely stored at-40to-80° Some viruses are rapidly inactivated by drying, others survive well in a desiccated state UV light inactivates viruses Enveloped viruses(lipid in envelope) are inactivated by organic solvents Phenols, alcohols Qacs active against viruses Most active agents are chlorine, hypochlorites, iodine, aldehydes. ethylene oxide
Antiviral Therapy-important notes 1. Virus replication is dependent to the host cells Antiviral must be selective for viral enzyme or protein, or inhibit virus-Specific process 2. Usually antivirals inhibit replication, dont kill virus Reliance on host immune response for ultimate virus elimination 3. High error rate of viral replication Rapid development of drug resistance Need to suppress virus replication rapidly and efficiently
1. Virus replication is dependent to the host cells Antiviral must be selective for viral enzyme or protein, or inhibit virus-specific process 2. Usually antivirals inhibit replication, don’t kill virus Reliance on host immune response for ultimate virus elimination 3. High error rate of viral replication Rapid development of drug resistance Need to suppress virus replication rapidly and efficiently Antiviral Therapy – important notes
ATTACHMENT Click after each step to view process PENETRATION HOST UNCOATING FUNCTIONS Transcription Translation REPLICATIO VIRAL 八 LIFE ASSEMBLY CYCLE ( MATURATION)、 RELEASE MULTIPLICATION
VIRAL LIFE CYCLE ATTACHMENT PENETRATION HOST FUNCTIONS ASSEMBLY (MATURATION) Transcription REPLICATION RELEASE UNCOATING Translation MULTIPLICATION Click after each step to view process
Potential sites of action for antiviral agents · Site of action attachment to the host cell uncoating of the viral genome nucleic acid synthesis assembly of progeny virions release of virus particles from host cell Drugs inhibit ongoing replication at host cell leve and replication will resume on removal of drug agents are not effective in elimination of non replicating or latent virus