Attachment 1. Using agents which imic the virus attachment protein (VAP)and bind to the No virus Entry cellular receptor Virus Entry At cepto ansbedy Aninon Cytoplasm
Attachment 2. Agents which mimic the receptor and bind to the vap Aeeeplo antit°y ntibody tibodY Roeeeto mimie Virus Entry No virus Entry ML「 Cytoplasm
Attachment Example: Fuzeon-anti-retroviral drug (Inhibition of gp41 mediated infusion) HⅣ F凵zE口n enfuvirtide Fuzeon CD4+ T Cell (enfuvirtide)
Example: Fuzeon – anti-retroviral drug (Inhibition of gp41 mediated infusion) Attachment Fuzeon (enfuvirtide)
HIV 9p41 gp120 gp120 CD4 CXCR4 CXCR4 CD4 gp41 CD4 1. gp120 binds to CD4 molecule 2. gp120 changes shape, peels back and binds to chemokine receptor 3. gp4 1 darts out and pierces cell membrane and anchors Virus 4. Fusion of membranes begins
Penetration/Uncoating Difficult to specifically target these stages of the life cycle as relatively little is known about them Pleconaril -blocks attachment uncoating of picornaviruses(eg rhinovirus) Amantadine rimantidine-? Block cellular membrane ion channels Drug treated cells unable to lower ph of the endosomal compartment- needed to allow influenza virus Ha protein to fuse to the cell membrane