GeneticEngineeringThese cells musthaveintactTgenesThese cells musthave intactPgenesand defective TgenesNext, these colonies are sub-cultured onto agar containingtetracycline
目录 Genetic Engineering Next, these colonies are sub-cultured onto agar containing tetracycline. These cells must have intact T genes These cells must have intact P genes and defective T genes
GeneticEngineeringThis colony will probably have the correct plasmid to produce the product from thehuman gene. Cells from this colony will be grown on a large scale and the mediumanalysed for the presence of the product from the human gene, eg growth hormone
目录 Genetic Engineering This colony will probably have the correct plasmid to produce the product from the human gene. Cells from this colony will be grown on a large scale and the medium analysed for the presence of the product from the human gene, eg growth hormone
（二）工具酶>限制性核酸内切酶>DNA聚合酶I>逆转录酶>T,DNA连接酶>碱性磷酸酶>末端转移酶>Taq DNA聚合酶目聚录
目录 （二）工具酶 ➢ 限制性核酸内切酶 ➢ DNA聚合酶Ⅰ ➢ 逆转录酶 ➢ T4DNA连接酶 ➢ 碱性磷酸酶 ➢ 末端转移酶 ➢ Taq DNA聚合酶
限制性核酸内切酶（restrictionendonuclease> 定义:限制性核酸内切酶(restriction endonucleaseRE)是识别DNA的特异序列，并在识别位点或其周围切割双链DNA的一类内切酶。Bam HIGGGATCCGATCC+GCCTAGCCTAGG目录
目录 限制性核酸内切酶(restriction endonuclease) 限制性核酸内切酶(restriction endonuclease, RE)是识别DNA的特异序列, 并在识别位点或其 周围切割双链DNA的一类内切酶。 GGATCC CCTAGG G CCTAG GATCC G + Bam HⅠ ➢ 定义：
>分类：I、I、I（基因工程技术中常用型）>作用：与甲基化酶共同构成细菌的限制修饰系统，限制外源DNA，保护自身DNA聚
目录 与甲基化酶共同构成细菌的限制修饰 系统，限制外源DNA，保护自身DNA。 Ⅰ、Ⅱ、Ⅲ（基因工程技术中常用Ⅱ型） ➢ 分类： ➢ 作用：