《生物化学与分子生物学》课程教学资源（课件讲稿）第23章 基因重组和基因工程（重组DNA技术 Recombinat DNA technology）

GeneticEngineeringNext the fragments are spread out and the required oneisolated.Fragmentwithrequiredgene

目录 Genetic Engineering Next the fragments are spread out and the required one isolated. Fragment with required gene

Genetic EngineeringCytoplasmPlasmidBacterial cell wallBacterial chromosomeStructure of a typical bacterium

目录 Genetic Engineering Cytoplasm Bacterial chromosome Bacterial cell wall Plasmid Structure of a typical bacterium

GeneticEngineeringPlasmidPlasmids are loops of DNA separate from the main chromosome.They carry genes for things like antibiotic resistance. This makesthem very useful to the Genetic engineer

目录 Genetic Engineering Plasmid Plasmids are loops of DNA separate from the main chromosome. They carry genes for things like antibiotic resistance. This makes them very useful to the Genetic engineer

GeneticEngineeringPIn the above plasmid, the blue gene is one that gives the bacteriumresistance to one antibiotic (eg Penicillin).The GREEN gene gives resistance to a different antibiotic (egTetracycline)

目录 Genetic Engineering In the above plasmid, the blue gene is one that gives the bacterium resistance to one antibiotic (eg Penicillin). P T The GREEN gene gives resistance to a different antibiotic (eg Tetracycline)

GeneticEngineeringPCuthereBy using special enzymes, we can make a cut in the midst ofONE of these antibiotic resistance genes.In this example, wewill cut opentheT'gene

目录 Genetic Engineering By using special enzymes, we can make a cut in the midst of ONE of these antibiotic resistance genes.In this example, we will cut open the ‘T’ gene P T Cut here