武汉大学Wuhan UnitStructural ProteomicsSeparationIdentificationIdentification of the PTM (post translationmodification)Comparison of theProteome in differentstates. (health/disease;drug treated/untreated)Laboratoryofplant molecular cytogenetics
Laboratory of plant molecular cytogenetics Structural Proteomics n Separation n Identification n Identification of the PTM (post translation modification) n Comparison of the Proteome in different states. (health/disease;drug treated/untreated)
武汉大学Wuhan UniversityStructuralProteomics High Throughputprotein structurex-raysdetermination via X-crystalray crystallography,detectorNMR spectroscopy orcomparativemolecular modelingLaboratory of plant molecular cytogenetics
Laboratory of plant molecular cytogenetics Structural Proteomics • High Throughput protein structure determination via Xray crystallography, NMR spectroscopy or comparative molecular modeling
Fig.6.Surface chargedistribution andfactorPore1Wallbinding sites,The sur-Pathforfaceof Pol lliscoloredstraightWallaccording to the elec-DNAtrostatic surface po-TFIIStential (84).with nega-tive,neutral,and posi-tive charges shown inred,white,andbluerespectively.Theactivesiteismarkedbyapinksphere.The asterisk in-dicatesthelocationofthe conserved start ofClampc-Amanitibottoma fragment of E.colifronttopRNA polymerase sub-Structural Basis ofunit βthat has beencross-linkedto anextrudedRNA3end (54)Transcription:RNA PolymeraseIl at 2.8 AngstromResolutionFig.7.Four mobileACPatrickCramer,*DavidA.Bushnell,RogerD.Kornbergmodules of thePol IIJnw-lobestructure.(A)Back-90°moduiebone traces of thecore.Jaw-lobe,clampand shelf modulesoftheform1structureshown ingray,blueyellow, and pink, re-spectively.(B)Chang-es in the position ofthe Jaw-lobe,clampCoreand shelf modulesbe-moduletween form 1 (col-shelfored)andform2madulestructures (gray).Thefrontfrontsidearrows indicate thedirection of chargesfromfom1tofom2Thecoremodules inthetwocrystalfomsweresuperimposedandthenomittedforclarity.(C)TheViewin(B)rotated9o°aboutaverticalaxicoreand law-lobemodulefAtheclaanbehweenitsedeand.thatotnalotwida
Laboratory of plant molecular cytogenetics
武汉大学Wuhan UniversityFunctional ProteomicsLocalization within the cellsProtein complexdeterminationProtein-ProteinInteraction /Interaction NetworkFunction the proteins involved in.(Metabolic and regulatory pathway;Signal transduction pathway)Laboratoryofplant molecular cytogenetics
Laboratory of plant molecular cytogenetics n Localization within the cells n Protein complex determination n Protein-Protein Interaction / Interaction Network n Function the proteins involved in. (Metabolic and regulatory pathway; Signal transduction pathway) Functional Proteomics
武汉大学WuhanUniversityFunctionalProteomicsGrowthfactorCellmembranePRSLSHp21rasPlakPaPSp21rasGDPGDPp110kinaseSosGrb2p85raspathwayRaf-1PPLCyDynaminDAG,MEK*PI4,5PPKC'scaMAPKNucleusTranscriptionc-FosC-Mycc-JunLaboratoryof plant molecular cytogenetics
Laboratory of plant molecular cytogenetics Functional Proteomics