Mixed InhibitorsUsuallyAffect Boththe Apparent Km and the VmaxVmaxisaffected(b)Vmbecausetheeffective[E] on which Vmax0.8dependsdecreases0.6Vo=20.4apparentKmmay=2α=40increaseordecrease00800km3204060100dependingonwhich[S] (μM)enzymeformtheinhibitorbindsmoststrongly
ALineweaver-BurkPlotRevealsMixedInhibition(c)()同+v=[T](uw/wr)linesintersecttothe leftof-150theyaxisNoinhibitor ()Nelson&Cox,LehningerPrinciplesofBiochemistry.e2021w.H.FreemanandCompay