Experiment1GOD-PODMethod forThe Estimation ofBloodGlucose
GOD-POD Method for The Estimation of Blood Glucose Experiment 1
1Introduction: Glucose is the major carbohydrate present in the blood.: It serves as a principal fuel for all the body tissues including the brainIt undergoes a series of chemical reactions to produce energy.·Accurate and precise measurement of blood glucose level is of greatimportance in the diagnosis and management of diabetes and otherdisorders of carbohydrate metabolism, hyperglycemia, andhypoglycemia
1 Introduction • Glucose is the major carbohydrate present in the blood. • It serves as a principal fuel for all the body tissues including the brain. It undergoes a series of chemical reactions to produce energy. • Accurate and precise measurement of blood glucose level is of great importance in the diagnosis and management of diabetes and other disorders of carbohydrate metabolism, hyperglycemia, and hypoglycemia
Glucose-oxidase Peroxidase (GOD POD) method: The most common method of glucose estimationEstimationofBloodGlucoseByGOD-PODMethod＃
Glucose-oxidase Peroxidase (GOD POD) method • The most common method of glucose estimation
2 Principle> In the presence of atmospheric oxygen, glucose present in thespecimen is oxidized by the enzyme glucose oxidase (GOD) togluconic acid and hydrogen peroxide (H,O2).> H,O, couples with 4-aminoantipyrine and phenol in presence ofperoxidase (POD) to form red-colored quinoneimine dye, which ismeasured colorimetrically at 540 nm> The intensity of the color is directly proportional to the concentrationofglucosepresentinthespecimenGlucoseOxidase(GOD)Glucose+HzO+O2Gluconicacid + HzO2Peroxidase (POD)Red quinoneimine dye +H20HzOz+Phenol+4-aminoantipyrine
2 Principle In the presence of atmospheric oxygen, glucose present in the specimen is oxidized by the enzyme glucose oxidase (GOD) to gluconic acid and hydrogen peroxide (H2O2). H2O2 couples with 4-aminoantipyrine and phenol in presence of peroxidase (POD) to form red-colored quinoneimine dye, which is measured colorimetrically at 540 nm. The intensity of the color is directly proportional to the concentration of glucose present in the specimen
3Reagents>Glucose standard>GOD-PODreagent:VEnzyme reagent mixture containing glucose oxidase (GOD), peroxidase(POD), 4-aminoantipyrine, phenol, and phosphate buffer (pH-7.0)some stabilizers and activators
3 Reagents Glucose standard GOD-POD reagent: Enzyme reagent mixture containing glucose oxidase (GOD), peroxidase (POD), 4-aminoantipyrine, phenol, and phosphate buffer (pH≈7.0), some stabilizers and activators