ALineweaver-BurkPlotRevealsCompetitive Inhibition(c)-(e)+[I]-3linesintersectattheyaxis10α=1NoinhibitorαKm1SlopeV.Vmax高()Neson&Cox,LehningerPrinciplesofBiochemisry22w.H.FreemanandCompany
UncompetitiveInhibitionuncompetitive(a)E+SESE+Pinhibitor:+1bindsatasiteSdistinctfromthesubstrate active siteESIunlikeacompetitiveinhibitor,binds onlytotheEScomplex-typeofreversibleNelson & Cox, Lehninger Principles of Biochemistry,Be, 0 2021 W.H, Freeman and Companyinhibition
Uncompetitive Inhibitors AffectBoththe Apparent Km and the Vmax. the measured Vmax(b)decreasesbecauseVmat high [S], Vo0.8approaches Vmax/a0.6Vo=20.4apparentKmr=30.2decreasesbecausethe [S] required to0.00km20406080100reach/Vmax[S] (um)decreases bytheNelson&Cowee2021Wtfactora
ALineweaver-BurkPlotRevealsUncompetitiveInhibition(c)-()+[1]α=2uwwm)αm1.5Llinesareparallel110a司 ()Nelson&Cox,LehningerPrinciplesofBiochemistry,e,2021W,H.FreemanandCompany
MixedInhibitionmixedinhibitor:(a)E+PE+SFS-bindsatasite+distinctfrom11Sthesubstrateactive siteEI+SESIbindstoeitherEortheEScomplextypeofNetson&CoxLehraerPrinciniesofaiocheristr:BeD2o21W.HFandComnreversibleinhibition