免疫电镜技术 ◆免疫铁蛋白技术 ◆免疫酶标技术 ◆免疫胶体金技术 应用:通过对分泌蛋白的定位,可以确定某种蛋白 的分泌动态;胞内酶的研究:膜蛋白的定位与骨架 蛋白的定位等 E
免疫电镜技术： 免疫铁蛋白技术 免疫酶标技术 免疫胶体金技术 应用：通过对分泌蛋白的定位，可以确定某种蛋白 的分泌动态；胞内酶的研究；膜蛋白的定位与骨架 蛋白的定位等 E
2)Scanning Electron microscopy(SEMS) The means to examine in great clarity and detail the surfaces of objects ranging in size from a virus to an animal head keeping surface properties as the living state It is very important to display the outer cell surface and various processes, extensions and extracellular materials that are involved in the interaction of cell--its environment
2) Scanning Electron microscopy (SEMs) The means to examine in great clarity and detail the surfaces of objects ranging in size from a virus to an animal head keeping surface properties as the living state. It is very important to display the outer cell surface and various processes, extensions and extracellular materials that are involved in the interaction of cell—its environment
SCANNING ELECTRON MICROSCOPY electron condenser lens beam deflector scan generator objective video lectrons from screen specimen detector acumen Figure 18.18 Scanning electron microscopy Scanning siosfromt A N. Bnens, B.L. Panessa, md F Gramm. Sonce Adunoement ef Sonne tr curtesy ef H F Heam mLE
iary axoneme from the protozoan Tetrahymena. The ar. a cil. Figure 18 17 Deep etching. Electron micrograph of onemes were fixed, frozen, and fractured, and the frozen water at the surface of the fractured block was evaporated away, leaving a portion of the axoneme standing out in re- lief, as visualized in this metal replica. The arrow indicates a distinct row of outer dynein arms (Fron Ursula W. Goodenough and John E. Heuser, J. Cell BioL. 95: 800, 1982, by copyright permission of the Rockefeller University Press
Liquid freon Liquid№z Figure 18 16 Freeze-fracture replicas. (a) Procedure for 8e..8.the formation of freeze-fracture replicas as described in the text Freeze-etching is an optional step in which a thin layer (b) Replica of a freeze-fractured onion root cell show- pores complex(G), a cytoplasmic vacuole (V), and the cell w Etching Replica viewed in and replicating