Based on the function and the chemical components Basic medium contains the basic nutrients for most of bacterial growth; the base of other kind of media e.g. broth e Nutrient Medium/Enriched Medium Additional or special nutrients(e. g, serum, growth factors, trace elements) are added to support some fastidious bacterial growth e.g. blood agar
11 --contains the basic nutrients for most of bacterial growth; --the base of other kind of media. --e.g. broth. Additional or special nutrients (e.g.,serum, growth factors, trace elements) are added to support some fastidious bacterial growth. e.g. blood agar. Based on the function and the chemical components: Basic Medium Nutrient Medium/Enriched Medium • l
Staphylococcus - blood agr plates Colonies of s, aureus with zone White colonies of S epidermidis of hemolysis on a blood agar plate on a blood agar plate 金黄色葡萄球菌在血平板上的 表皮葡萄球菌在血平板上的 金黄色菌落及溶血环 白色菌落
Colonies of with zone of hemolysis on a blood agar plate White colonies of on a blood agar plate S. aureus S. epidermidis Staphylococcus – blood agr plates 金黄色葡萄球菌在血平板上的 金黄色菌落及溶血环 表皮葡萄球菌在血平板上的 白色菌落
Dewdrop-like colonies of Neisseria meningitidis on a chocolate plate 脑膜炎双球菌在巧克力平板上的露滴状菌落
Dewdrop-like colonies of on a chocolate plate Neisseria meningitidis 脑膜炎双球菌在巧克力平板上的露滴状菌落
Selective mediun the medium that can prevent the certain bacterial growth while permitting others. e.g. SS agar(Salmonella Shigella agar) Differential medium Escherichia cofr Some special substrates and indicators are added into the media in order to produce a visual differentiation when several bacteria grow on the same kind of medium e.g. Kligler Iron Agar pH indicator: phenol red Slant: containing lactose Slant agar of Kligler Iron Agar Butt: semisolid 克氏双糖铁斜面 containing glucose and Ferrous ammonium sulfate
14 the medium that can prevent the certain bacterial growth while permitting others. e.g. SS agar (Salmonella Shigella Agar) Some special substrates and indicators are added into the media in order to produce a visual differentiation when several bacteria grow on the same kind of medium. e.g. Kligler Iron Agar • • Selective Medium Differential Medium Butt: semisolid, containing glucose and Ferrous ammonium sulfate Slant: containing lactose pH indicator: phenol red Slant agar of Kligler Iron Agar 克氏双糖铁斜面
Red slant indicates Yellow slant no lactose ← indicates lactose metabolism metabolism Black coloration indicates reduction of sulfur and Yellow butt indicates production of H,s glucose metabolism and gas formation; Yellow butt indicates Clouding semisolid t Glucose metabolism agar indicates motility Clouding semisolid agar indicates motility Bacillus proteus growth in Kligler Iron Agar E coli growth in Kligler Iron Agar 变形杆菌在双糖铁培养基上的生长情况 大肠埃希菌在双糖铁培养基上的生长情况
Bacillus proteus growth in Kligler Iron Agar E.coli Black coloration indicates reduction of sulfur and production of H2S Yellow butt indicates Glucose metabolism; Clouding semisolid agar indicates motility Red slant indicates no lactose metabolism growth in Kligler Iron Agar Yellow butt indicates glucose metabolism and gas formation; Clouding semisolid agar indicates motility Yellow slant indicates lactose metabolism 变形杆菌在双糖铁培养基上的生长情况 大肠埃希菌在双糖铁培养基上的生长情况