BIOCHEMISTRYANDMOLECULARBIOLOGYATPAMPR-5-PPP-1-R-5-PRibose phosphate ( ribose 5-phosphate )(5-phosphoribosyl1.pyrophosphate)pyrophosphokinaseGlutamineGlutamine:phosphoribosylGlutamatePyrophosphateamidotransferaseH,N-1-R-5'-PGlutamine,glycine,onecarbon(5'-phosphoribosylamine)unit,CO2 and aspartate1participatein reaction1respectively.11AMP￥IMPGMPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY R-5-P （ ribose 5-phosphate ） AMP ATP Ribose phosphate pyrophosphokinase PP-1-R-5-P （ 5-phosphoribosyl 1- pyrophosphate ） Glutamine,glycine,one carbon unit, CO2 and aspartate participate in reaction respectively. IMP AMP GMP H2N-1-R-5´-P （5´-phosphoribosylamine） Glutamine Glutamate Glutamine: phosphoribosyl Pyrophosphate amidotransferase
BIOCHEMISTRYANDMOLECULARBIOLOGY1. Synthesis of IMPPRPPNHa*HNHNGlnCH2GlyADPNIO.CH2GlnCH2N++Formyl-Glu++Glu+PP,O-CATPP0=THFTHFHN-CATPADP+P,L1-111NH2NHNHNH??0Ribose-PRibose-PRibose-PRibose-P5'-Phosphoribosyl5'-PhosphoribosylN-5'-Phosphoribosylamine5'-PhosphoribosylglvcinamideN-formylglycinamideformvlglvcinamidine(PRA)(GAR)(FGAR)(FGAM)ATPADP+①Glutamine:phosphoribosylPyrophosphateamidotransferaseHCCH②GARSynthetaseH2N-③FormyltransferaseRibose-P@Synthetase5'-Phosphoribosyl(AIR)5-aminoimidazole@SynthetaseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ① Glutamine:phosphoribosyl Pyrophosphate amidotransferase ② GAR Synthetase ③ Formyltransferase ④ Synthetase ⑤ Synthetase 1. Synthesis of IMP
OADPAsp'tHATPPO0C-C-N-HCH2OOCH.NCarboxylaseSynthetaseRibose-PRibose-pRibose-P5'-Phosphoribosyl5'-Phosphoribosy4-5'-PhosphoribosylN-succinocarboxamide5-aminoimidazole 4-5-aminoimidazole5-aminoimidazolecarboxylate(AIR)(SAICAR)(GAIR)CyclohydrolaseFumarateAdenylosuccinatelyaseO0ON9:Formyl-THETHFH2NH2N109FormyltransferaseHRibose:pRibose-PRibose-p5'-Phosphoribosy14-5'-Phosphoribosyl4-Inosine5'-monophosphatecarboxamide 5-carboxamide 5-(IMP)formamidoimidazoleaminoimidazole(ARCAR)(FAICAR)
2.Synthesis of AMP and GMPFumarateHOOC-CH2COO延胡索酸NHNH2GTPGDP+PASP?天冬氨酸Rib-PRib-P1AMP腺苷酸代琥珀酸AdenylosuccinateRib-H,ONAD*IMPNADH+H*GlnGluATPAMP+PP,?HNHN0H.NH,0Rib-Rib?GMPXMPDAdenylosuccinatesynthetaseIMPdehydrogenase②Adenylosuccinase@GMPsynthetase
①Adenylosuccinate synthetase ③IMP dehydrogenase ②Adenylosuccinase ④GMP synthetase ASP Fumarate Adenylosuccinate 2. Synthesis of AMP and GMP
ABIOCHEMISTRYANDMOLECULARBIOLOGYKinaseKinaseATPAMPADPATPADPATPADPKinaseKinaseGMPGDPGTPADPATPATPADPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY AMP ADP ATP ATP ADP Kinase ATP ADP Kinase GMP GDP GTP ATP ADP Kinase ATP ADP Kinase