BIOCHEMISTRYANDMOLECULARBIOLOGYSynthesis pathway of purine nucleotideDe novo synthesis pathwaySalvage synthesis pathwayDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY De novo synthesis pathway Salvage synthesis pathway Synthesis pathway of purine nucleotide
BIOCHEMISTRYANDMOLECULARBIOLOGYA. De novo purine nucleotide synthesis·DefinitionThe atoms of the purine ring are contributed by anumber of compounds, including amino acid(asparticacid, glycine, and glutamine), CO2, and derivatives oftetrahydrofolate.·SynthesisorganThe major organ for synthesis purine nucleotide is liver,next organ is intestine and thymus gland, but brain and bonemarrow can not use de novo to synthesize purine nucleotide.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY The atoms of the purine ring are contributed by a number of compounds, including amino acid(aspartic acid, glycine, and glutamine), CO2 , and derivatives of tetrahydrofolate. The major organ for synthesis purine nucleotide is liver， next organ is intestine and thymus gland，but brain and bone marrow can not use de novo to synthesize purine nucleotide. •Definition •Synthesis organ A. De novo purine nucleotide synthesis
ABIOCHEMISTRYANDMOLECULARBIOLOGYSources of the individual atoms in thepurine ringCO2Glycine1CNAspartateNiCN10-Formy!-CBtetrahydrofolateN10-Formyl-（Onecarbonunit)CNgtetrahydrofolateN3(One carbon unit)1→GlutamineDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY CO2 Aspartate N10-Formyltetrahydrofolate （One carbon unit) Glycine N10 -Formyltetrahydrofolate （One carbon unit） Glutamine Sources of the individual atoms in the purine ring
沙BIOCHEMISTRYANDMOLECULARBIOLOGYMaterial GlycineGlycine in the middleCO2The top is CO2 AspartateLeft up is Aspartate GlutamineGlutamine and oneOne carbon unitcarbon unit in the two side5'-Phosphate riboseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Material • Glycine • CO2 • Aspartate • Glutamine • One carbon unit • 5’-Phosphate ribose Glycine in the middle The top is CO2 Left up is Aspartate Glutamine and one carbon unit in the two side
BIOCHEMISTRYANDMOLECULARBIOLOGYPathway1. Synthesis of IMP2. Synthesis of AMP and GMPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY 1. Synthesis of IMP 2. Synthesis of AMP and GMP Pathway