BIOCHEMISTRYANDMOLECULARBIOLOGYCharacteristic of De novo synthesis of purinenucleotidePurine nucleotide is synthesis in 5'- ribosephosphate from step to step.Synthesis of IMP need to 5 ATP and 6 high energyphosphate bond.Synthesis of AMP or GMP need one more ATP.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY •Purine nucleotide is synthesis in 5’- ribose phosphate from step to step. Synthesis of IMP need to 5 ATP and 6 high energy phosphate bond. Synthesis of AMP or GMP need one more ATP. Characteristic of De novo synthesis of purine nucleotide
The regulation of de novo synthesisRegulation mode: feedback regulation and cross regulation+Adenylo-→AMPADP-→ATPRibosephosphateR-5-RPyrophospheGlutamine:PRPPRA→-IMPkinasesuccinatephosphorrbosylATPPyrophosphatt-XMP+GMIPGDP-GTPamidotransferaseAdenylo--AMP*ADP*ATPsuccinate1GTPIMPtGMP+GDP GTPXMP福ATP
R-5-P ATP Ribose phosphate Pyrophosphokinase PRPPGlutamine: phosphoribosyl Pyrophosphate amidotransferase PRA IMP Adenylosuccinate AMP ADP ATP XMP GMP GDP GTP + + _ _ _ _ _ IMP Adenylosuccinate XMP AMP ADP ATP GMP GDP GTP ATP GTP _ _ + + Regulation mode：feedback regulation and cross regulation The regulation of de novo synthesis
北BIOCHEMISTRYANDMOLECULARBIOLOGYB.Salvage pathway for purine nucleotide·DefinitionPurines that result from the normal turnoverof cellular nucleic acids or that are obtained fromthe diet and not degraded can be reconverted intonucleoside triphosphates and used by the bodyDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Purines that result from the normal turnover of cellular nucleic acids or that are obtained from the diet and not degraded can be reconverted into nucleoside triphosphates and used by the body. •Definition B. Salvage pathway for purine nucleotide