BIOCHEMISTRYANDMOLECULARBIOLOGYOHCCACCAend* The secondary of tRNAGeCloverleaf patternAminoDLUacid armQ00DHUCC-GUC> amino acid armloopAUCCCCCCADDG> DHU loop1GGGGGCGm1> anticodon loopPGCAAGA2> variableGATyCloopUUC> TYC loopACAUGanticodonloopDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY * The secondary of tRNA—— Cloverleaf pattern ➢ amino acid arm ➢ DHU loop ➢ anticodon loop ➢ variable ➢ TΨC loop DHU loop CCA end Amino acid arm TΨC loop anticodon loop
BIOCHEMISTRYANDMOLECULARBIOLOGY氨基酸mino acid arm3端* Tertiary structure of tRNA-InverseLshape·Function of tRNAActivelycarry amino acid toribosome, participate in synthesisAnticodonof protein反密码子Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY * Tertiary structure of tRNA— — Inverse L shape •Function of tRNA Actively carry amino acid to ribosome, participate in synthesis of protein Amino acid arm Anticodon
BIOCHEMISTRYANDMOLECULARBIOLOGY4.The Structure and Function of ribosomeRNA* The structure ofrRNA*ofrRNAFunctioninformingParticipateforribosomeproteinsynthesisDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY 4. The Structure and Function of ribosome RNA * The structure of rRNA * Function of rRNA Participate in forming ribosome for protein synthesis
TABIOCHEMISTRYANDMOLECULARBIOLOGY* Classification of rRNA (sedimentation coefficient)EukaryoteProkaryote5S rRNA5S rRNA28SrRNA23SrRNA16S rRNA5.8S rRNA18S rRNADepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY * Classification of rRNA（sedimentation coefficient） Eukaryote 5S rRNA 28S rRNA 5.8S rRNA 18S rRNA Prokaryote 5S rRNA 23S rRNA 16S rRNA
ABIOCHEMISTRYANDMOLECULARBIOLOGYComponent of ribosomeProkaryote (E.coli)Eukaryote(Mouse liver)Small30S40Ssubunit16S18SrRNA1874nucleotides1542 nucleotides2133Protein40% weight50% weightspeciesspecies50S60SLargesubunit23S28SrRNA2940 nucleotides4718 nucleotides5S5.85S120 nucleotides160 nucleotides5S120 nucleotides3149Protein30% weight35% weightspeciesspeciesDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Component of ribosome Prokaryote（E.coli） Eukaryote（Mouse liver） Small subunit 30S 40S rRNA 16S 1542 nucleotides 18S 1874 nucleotides Protein 21 species 40% weight 33 species 50% weight Large subunit 50S 60S rRNA 23S 5S 2940 nucleotides 120 nucleotides 28S 5.85S 5S 4718 nucleotides 160 nucleotides 120 nucleotides Protein 31 species 30% weight 49 species 35% weight