13.2 DNA polymerases are the enzymes that make DNA 5 3 3'l 8 Re8te088gee 5 Figure 13.2 Repair synthesis replaces a damaged strand of DNA 清菜大当
Figure 13.2 Repair synthesis replaces a damaged strand of DNA. 13.2 DNA polymerases are the enzymes that make DNA
Template has free 3'-OH end 13.2 DNA polymerases 5.PP PP OH3 are the enzymes that make DNA 3'mn Incoming nucleotide has 5'-triphosphate Figure 13.3 DNA synthesis 5'PPR OH3'3' occurs by adding nucleotides to the 3 -OH end of the growing 5am.PPP2P、OH3 chain,so that the new chain is synthesized in the 5 -3 3'n 经P3经025 direction.The precursor for DNA Diphosphate is released when synthesis is a nucleoside nucleotide is added to chain triphosphate,which loses the 5P PPPP POH terminal two phosphate groups in the reaction. 3 清菜大当
Figure 13.3 DNA synthesis occurs by adding nucleotides to the 3 -OH end of the growing chain, so that the new chain is synthesized in the 5 -3 direction. The precursor for DNA synthesis is a nucleoside triphosphate, which loses the terminal two phosphate groups in the reaction. 13.2 DNA polymerases are the enzymes that make DNA
13.2 DNA polymerases are the enzymes that make DNA DNA polymerases in E.coli poiA ma jor repair enzyme poiB min or repair enztme 用 poiC rep licase IV dinB SOS repair umuD'C SOS repair Figure 13.4 Only one DNA polymerase is the replicase The others participate in repair of damaged DNA 清菜大当
Figure 13.4 Only one DNA polymerase is the replicase. The others participate in repair of damaged DNA. 13.2 DNA polymerases are the enzymes that make DNA
13.3 DNA polymerases have various nuclease activities Nick translation describes the ability of E.coli DNA polymerase I to use a nick as a starting point from which one strand of a duplex DNA can be degraded and replaced by resynthesis of new material: is used to labeled nucleotides into DNA vitro 清菜大当
Nick translation describes the ability of E. coli DNA polymerase I to use a nick as a starting point from which one strand of a duplex DNA can be degraded and replaced by resynthesis of new material; is used to introduce radioactively labeled nucleotides into DNA in vitro. 13.3 DNA polymerases have various nuclease activities
13.4 DNA polymerases control the fidelity of replication Proofreading refers to any mechanism for correcting errors in protein or nucleic acid synthesis that involves scrutiny of individual units after they have been added to the chain 清菜大当
Proofreading refers to any mechanism for correcting errors in protein or nucleic acid synthesis that involves scrutiny of individual units after they have been added to the chain. 13.4 DNA polymerases control the fidelity of replication