Chapter 23 Catalytic RNA 清革大当
Chapter 23 Catalytic RNA
23.1 Introduction 23.2 Group I introns undertake self-splicing by transesterification 23.3 Group I introns form a characteristic secondary structure 23.4 Ribozymes have various catalytic activities 23.5 Some introns code for proteins that sponsor mobility 23.6 The catalytic activity of RNAase P is due to RNA 23.7 Viroids have catalytic activity 23.8 RNA editing occurs at individual bases 23.9 RNA editing can be directed by guide RNAs 清莘大当
23.1 Introduction 23.2 Group I introns undertake self-splicing by transesterification 23.3 Group I introns form a characteristic secondary structure 23.4 Ribozymes have various catalytic activities 23.5 Some introns code for proteins that sponsor mobility 23.6 The catalytic activity of RNAase P is due to RNA 23.7 Viroids have catalytic activity 23.8 RNA editing occurs at individual bases 23.9 RNA editing can be directed by guide RNAs
23.1 Introduction The idea that only proteins have enzymatic activity was deeply rooted in biochemistry. The enzyme ribonuclease P is a ribonucleoprotein that contains a single RNA molecule bound to a protein. Small RNAs of the virusoid class have the ability to perform a self-cleavage reaction. Introns of the group I and group II classes possess the ability to splice themselves out of the pre-mRNA that contains them. 清菜大兰
The idea that only proteins have enzymatic activity was deeply rooted in biochemistry. The enzyme ribonuclease P is a ribonucleoprotein that contains a single RNA molecule bound to a protein. Small RNAs of the virusoid class have the ability to perform a self-cleavage reaction. Introns of the group I and group II classes possess the ability to splice themselves out of the pre-mRNA that contains them. 23.1 Introduction
23.1 Introduction The common theme of these reactions is that the RNA can perform an intramolecular or intermolecular reaction that involves cleavage or joining of phosphodiester bonds in vitro. RNA splicing is not the only means by which changes can be introduced in the informational content of RNA. 情菜大当
The common theme of these reactions is that the RNA can perform an intramolecular or intermolecular reaction that involves cleavage or joining of phosphodiester bonds in vitro. RNA splicing is not the only means by which changes can be introduced in the informational content of RNA. 23.1 Introduction
23.2 Group I introns undertake self- splicing by transesterification Exon 1 Intron Exon 2 N八N入入八入NN Figure 23.1 Splicing of the Tetrahymena 35S rRNA precursor Gel electrophoresis Transcripti can be followed by gel 35S RNA electrophoresis.The removal of the intron is revealed by the Splicing appearance of a rapidly moving small band.When the intron becomes circular,it Cyclization electrophoreses more slowly,as seen by a higher band Circular intron Unear intron 清苇大当
Figure 23.1 Splicing of the Tetrahymena 35S rRNA precursor can be followed by gel electrophoresis. The removal of the intron is revealed by the appearance of a rapidly moving small band. When the intron becomes circular, it electrophoreses more slowly, as seen by a higher band. 23.2 Group I introns undertake selfsplicing by transesterification