BIOCHEMISTRYANDMOLECULARBIOLOGYRibonucleotideRibonucleosideordeoxyribonucleosideNH2bind phosphate by phosphate ester bond toform nucleotideOHO-PHO-CHNucleotide:OHAMP, GMP, UMP, CMPDeoxynucleotide:OH OHdAMP, dGMP, dTMP, dCMPDepartment of Biochemistryand Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY P O O OH HO O C H2 OH OH N N NH2 HO C H2 O O OH OH N N NH2 O Nucleotide： AMP, GMP, UMP, CMP Deoxynucleotide： dAMP, dGMP, dTMP, dCMP Ribonucleotide Ribonucleoside or deoxyribonucleoside bind phosphate by phosphate ester bond to form nucleotide
NHNH2OHBIOCHO三ODLECULARBIOLOGY5'endCO5'gThe linkage of nucleotide3NHOHHO-P三O5Nucleotide linkby5/CHa3',5'-phosphodiester bond toOHform nucleic acidHO-P=ONHGNH233endDepartment of Blochmistry and MoHular Biology, Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY 5´end 3´end The linkage of nucleotide Nucleotide link by 3’,5’-phosphodiester bond to form nucleic acid C G A
BIOCHEMISTRYANDMOLECULARBIOLOGYThemethod forwritingGTGTCA5'POH.3°15' pApCpTpGpCpT-oH 3'5'ACTGCT3Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY A G 5 P P T P G P C P T P OH 3 5 pApCpTpGpCpT-OH 3 5 A C T G C T 3 The method for writing
BIOCHEMISTRYANDMOLECULARBIOLOGYDimensionalStructureand Function ofDNAl.The secondary structure ofDNA--DoublehelixDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Dimensional Structure and Function of DNA 1.The secondary structure of DNA——Double helix
BIOCHEMISTRYANDMOLECULARBIOLOGYThebackground ofDNAdoublehelix structureBase component analysisChargaff rule: [A] = [T][G] = [C] The data of base analysisindicates the best way is A-T,G-C pairing with hydrogen bond>X-ray diffraction of fibrousDNA maps analysisDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Base component analysis Chargaff rule：[A] = [T] [G] [C] The data of base analysis indicates the best way is A-T、 G-C pairing with hydrogen bond X-ray diffraction of fibrous DNA maps analysis The background of DNA double helix structure